Suzuki T, Makino-Tasaka M
Anal Biochem. 1983 Feb 15;129(1):111-9. doi: 10.1016/0003-2697(83)90059-3.
A sensitive analytical method was developed in order to study the rhodopsin-porphyropsin system in the eye. Oximes of 11-cis-retinal, all-trans-retinal, 11-cis-3-dehydroretinal, and all-trans-3-dehydroretinal were determined quantitatively by high-pressure liquid chromatography. This method was applied to the analysis of retinal and 3-dehydroretinal in the retinas of bullfrog and goldfish. The results agreed with those obtained from the bleaching kinetics of visual pigment extracted with detergent. A reliable result is obtained if the tissue contains more than 5 pmol of retinal (or 3-dehydroretinal). The chromophore composition could be determined in the eye of a small freshwater prawn, Palaemon pancidence, using 50 pmol of 11-cis-retinal and no 3-dehydroretinal.
为了研究眼睛中的视紫红质 - 视紫蓝质系统，开发了一种灵敏的分析方法。通过高压液相色谱法定量测定了11 - 顺式视黄醛、全反式视黄醛、11 - 顺式 - 3 - 脱氢视黄醛和全反式 - 3 - 脱氢视黄醛的肟。该方法应用于牛蛙和金鱼视网膜中视黄醛和3 - 脱氢视黄醛的分析。结果与用去污剂提取的视觉色素的漂白动力学结果一致。如果组织中视黄醛（或3 - 脱氢视黄醛）含量超过5 pmol，就能得到可靠的结果。使用50 pmol的11 - 顺式视黄醛且不含3 - 脱氢视黄醛，可测定小型淡水虾罗氏沼虾眼睛中的发色团组成。
