用于单克隆抗体140.240放射性碘化的碘甘醚法与微电化学技术的比较
Comparison of the iodogen and the microelectrochemical techniques for the radioiodination of monoclonal antibody 140.240.
作者信息
Wong Z M, Teare F W, Bowen B M, Liao S K, Kwok C S, Boxen I
机构信息
University of Toronto, Faculty of Pharmacy, Ontario, Canada.
出版信息
Int J Rad Appl Instrum B. 1988;15(5):505-9. doi: 10.1016/s0969-8051(88)80006-4.
The Iodogen technique was compared with the microelectrochemical technique for the radioiodination of submilligram quantities of monoclonal antibody 140.240. The titre of the antibody against cultured melanoma cells was decreased five-fold after iodination with 0.80-0.85 atoms of iodine per molecule of antibody with both techniques. The Iodogen technique resulted in 82.1% incorporation of iodine into the antibody, somewhat higher than the 78.0% obtained with the microchemical technique (P = 0.10). Therefore, the Iodogen technique is the preferred method based on efficiency and convenience.
将碘珠法与微电化学技术用于亚毫克量单克隆抗体140.240的放射性碘化。两种技术在用每分子抗体0.80 - 0.85个碘原子碘化后，该抗体针对培养的黑色素瘤细胞的效价均降低了五倍。碘珠法使碘掺入抗体的比例为82.1%，略高于微化学技术的78.0%（P = 0.10）。因此，基于效率和便利性，碘珠法是首选方法。
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