Karatzas N D, Kallaras K I, Heal A V
Aristotelion University, Thessaloniki, Greece.
Int J Rad Appl Instrum B. 1988;15(5):525-8. doi: 10.1016/s0969-8051(88)80009-x.
Estimation of red blood cell volume (RBCV) and blood volume (BV) in experimental animals is important for studies concerning pharmaceutical distribution. In this study RBCV was measured, using 99mTc, in 64 male albino New Zealand rabbits with a body weight (BW) of 3.12 +/- 0.37 (SD) kg. The packed cell volume (PCV) was 38.17 +/- 2.37% (SD), the hematocrit (Hct) of the venous blood sample, corrected for trapped plasma, was PCV x 0.97 and the somatic hematocrit (Ho) was calculated as 0.89 x Hct. RBCV was measured using the pretinning method and the BV was calculated according to the formula BV = RBCV x 100/Ho. Labeling efficiency was 96.4% +/- 3.8% (SD). The RBCV was found to be 18.52 +/- 1.96 (SD) mL/kg (BW) and BV 56.12 +/- 4.82 (SD)mL/kg (BW). The correlation of the RBCV and BV to BW is given by the formulae: RBCV = 66.754 ln(BW(g)) - 478.702 (r2 = 0.624, P less than 0.001) and BV = -47.587 + 197.342 ln(BWkg) (r2 = 0.72, P less than 0.001). The 99mTc results do not differ significantly from those of the standard 51Cr procedure, but the 99mTc labeling method permits repetitive measurements at shorter intervals compared to 51Cr.
在实验动物中估计红细胞体积（RBCV）和血容量（BV）对于药物分布研究很重要。在本研究中，使用99mTc对64只体重为3.12±0.37（标准差）kg的雄性白化新西兰兔测量了RBCV。血细胞比容（PCV）为38.17±2.37%（标准差），静脉血样本的血细胞比容（Hct）经截留血浆校正后为PCV×0.97，体细胞血细胞比容（Ho）计算为0.89×Hct。使用预锡法测量RBCV，并根据公式BV = RBCV×100/Ho计算BV。标记效率为96.4%±3.8%（标准差）。发现RBCV为18.52±1.96（标准差）mL/kg（体重），BV为56.12±4.82（标准差）mL/kg（体重）。RBCV和BV与体重的相关性由以下公式给出：RBCV = 66.754 ln（体重（g）） - 478.702（r2 = 0.624，P＜0.001）和BV = -47.587 + 197.342 ln（体重（kg））（r2 = 0.72，P＜0.001）。99mTc的结果与标准51Cr程序的结果没有显著差异，但与51Cr相比，99mTc标记方法允许在更短的间隔内进行重复测量。
