Ulloa-García Armando, Dzul-Rosado Karla, Bermúdez-Castillero Sergio E, López-López Noé, Torres-Monzón Jorge Aurelio
Facultad de Ciencias Químicas, Campus IV, Universidad Autónoma de Chiapas. Tapachula, Chiapas, México.
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán. Mérida, Yucatán, México.
Salud Publica Mex. 2020 Jul-Aug;62(4):358-363. doi: 10.21149/10160.
To determine the presence of Rickettsia typhi in Rhipicephalus sanguineus s.l. and Amblyomma mixtum in southern Mexico.
Ticks were collected in humans and domestic animals. The presence of Rickettsia was determined by PCR and sequencing.
10/39 work vials amplified fragments of the gltA, htrA and ompB genes. On 7/10 from Rh. sanguineus s.l. collected from dogs and in 3/10 of A. mixtum collected from horse and human. Sequencing indicated R. typhi in Rh. sanguineus and A. mixtum with 100% homology (LS992663.1) for a region of the htrA gene and 99% (LS992663.1) with the regions of the gltA and OmpB genes. The minimum infection rate (TMI) for R. typhi was 3.88.
Rhipicephalus sanguineus s.l. and Amblyomma mixtum are naturally infected with R. typhi in Southern Mexico.
确定墨西哥南部血红扇头蜱指名亚种和混色花蜱中是否存在伤寒立克次体。
从人类和家畜身上采集蜱虫。通过聚合酶链反应（PCR）和测序确定立克次体的存在。
39个工作样本中有10个扩增出了gltA、htrA和ompB基因片段。其中，7个来自狗身上采集的血红扇头蜱指名亚种，3个来自马和人身上采集的混色花蜱。测序表明，血红扇头蜱和混色花蜱中的伤寒立克次体与htrA基因区域具有100%的同源性（LS992663.1），与gltA和OmpB基因区域具有99%的同源性（LS992663.1）。伤寒立克次体的最低感染率（TMI）为3.88。
在墨西哥南部，血红扇头蜱指名亚种和混色花蜱自然感染了伤寒立克次体。
