Singh Deepika, Debnath Pratima, Sane Aniruddha P, Sane Vidhu A
Plant Gene Expression Lab, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
Integral University, Kursi Road, Lucknow, 226026 India.
Physiol Mol Biol Plants. 2020 Jun;26(6):1187-1199. doi: 10.1007/s12298-020-00820-3. Epub 2020 May 14.
WRKY transcription factors (TFs) are a large plant-specific family of TFs that govern development and biotic/abiotic stress responses in plants. We have identified as a gene primarily expressed in roots. encodes a protein of 320 amino acids that functions as a transcriptional activator. It is transcriptionally up-regulated by ethylene, BAP and salicylic acid treatment but suppressed by IAA. Expression of in transgenic Arabidopsis affects sensitivity of roots to ethylene, JA and auxin with transgenic plants showing hypersensitivity to ethylene, JA and auxin-mediated primary root growth inhibition. This hypersensitivity is correlated with higher expression of and that mediate responses to these hormones. expression also affects aerial growth with transgenic plants showing greater number of leaves but smaller rosettes. Flowering time is reduced in transgenic lines and these plants also show a greater number of inflorescence branches, siliques and seeds. The siliques are longer and compactly packed with seeds but seeds are smaller in size. Root biomass shows a 25% decrease in transgenic Arabidopsis plants at harvest compared with controls. The studies show that regulates plant growth possibly through modulation of genes controlling hormone responses.
WRKY转录因子是植物特有的一个大型转录因子家族，在植物发育以及生物/非生物胁迫响应过程中发挥调控作用。我们已鉴定出一个主要在根中表达的基因。该基因编码一个由320个氨基酸组成的蛋白质，其功能为转录激活因子。经乙烯、BAP和水杨酸处理后，该基因转录上调，但受吲哚乙酸抑制。在转基因拟南芥中该基因的表达影响根对乙烯、茉莉酸和生长素的敏感性，转基因植株对乙烯、茉莉酸和生长素介导的主根生长抑制表现出超敏反应。这种超敏反应与介导对这些激素响应的该基因及其他基因的高表达相关。该基因的表达也影响地上部分生长，转基因植株叶片数量更多，但莲座叶丛更小。转基因株系的开花时间提前，这些植株还表现出更多的花序分支、角果和种子。角果更长且种子排列紧密，但种子尺寸更小。与对照相比，收获时转基因拟南芥植株的根生物量减少了25%。这些研究表明，该基因可能通过调控控制激素响应的基因来调节植物生长。
