CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Gandra, PRD, Portugal.
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Universidade do Porto, Edifício do Terminal de Cruzeiros do Porto de Leixões, Matosinhos, Portugal.
Crit Rev Anal Chem. 2021;51(8):787-811. doi: 10.1080/10408347.2020.1777522. Epub 2020 Jun 18.
Analytical methodologies to accurate quantify enantiomers are nowadays imperative in different areas such as pharmaceutical, agrochemicals, forensic toxicology, food and environmental analysis, among others. This review aims to discuss the use of gas chromatography (GC) methods as a tool for enantiomeric separations. The separation of enantiomers by GC methodologies has been performed using both direct and indirect methods, nevertheless, the selected methodology depends not only on the physical chemical properties of the target compounds but also on the field of application. Indeed, enantiomeric separation by GC in the forensic toxicology field was frequently performed using indirect methods for pharmaceuticals and illicit drugs, while direct methods have been reported as the preferable choice for pesticides and polychlorinated biphenyl compounds. Concerning food analysis chiral separation have been mainly carried out by direct methods while for environmental analysis, both direct and indirect methods have been reported, depending on the class of compounds studied. This manuscript compiles analytical methods of enantioseparation by GC, both direct and indirect methods, and its applicability in forensic toxicology, food and environmental analysis.
目前，在制药、农化、法医毒理学、食品和环境分析等多个领域，准确量化对映异构体的分析方法至关重要。本文旨在讨论气相色谱（GC）方法作为对映异构体分离工具的应用。通过 GC 方法对映异构体的分离采用了直接和间接两种方法，但所选方法不仅取决于目标化合物的物理化学性质，还取决于应用领域。实际上，在法医毒理学领域中，通过 GC 对药物和非法药物进行对映异构体分离时，通常采用间接方法，而对于农药和多氯联苯化合物，则报道了直接方法作为首选。在食品分析中，手性分离主要通过直接方法进行，而对于环境分析，根据所研究的化合物类别，报道了直接和间接方法。本文汇编了 GC 对映异构体分离的分析方法，包括直接和间接方法，及其在法医毒理学、食品和环境分析中的应用。
