Wang Huan, Hu Chunyan, Song Xiaole, Hu Li, Li Wanpeng, Yu Hongmeng, Sun Xicai, Wang Dehui
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.
Department of Clinical Pathology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.
Am J Rhinol Allergy. 2020 Nov;34(6):800-809. doi: 10.1177/1945892420930976. Epub 2020 Jun 17.
It was recently suggested that the upregulation of podoplanin (PDPN) in cancer cells plays a significant role in tumor invasion and metastasis and that it is significantly associated with poor prognosis in oral, cutaneous, and esophageal squamous cell carcinoma. The aim of this study was to investigate the expression pattern of PDPN in sinonasal squamous cell carcinoma (SNSCC) and to evaluate its role as a prognostic factor for survival outcome.
This study included 59 subjects with SNSCC. We retrospectively collected the clinical features of these patients from medical records and retrieved the associated formalin-fixed, paraffin-embedded tissues for PDPN immunohistochemical staining. Furthermore, PDPN expression was analyzed in relation to the patients' clinicopathological features and prognosis.
We observed positive staining for PDPN in both cancer cells and stromal cancer-associated fibroblasts (CAFs). Positive expression of PDPN in cancer cells of patients with SNSCC was significantly correlated with the primary tumor site (p = 0.009) and local recurrence (p = 0.024). In addition, patients with PDPN-positive cancer cells had significantly lower overall survival (OS) and disease-free survival (DFS) rates than did patients with PDPN-negative cancer cells (both p < 0.05). Multivariate analysis revealed that PDPN expression in cancer cells was an independent prognostic factor for both OS (p = 0.038) and DFS (p = 0.039).
Our findings demonstrated that PDPN overexpression may be both an independent prognostic biomarker and a therapeutic target in SNSCC.
最近有研究表明，癌细胞中血小板反应蛋白-1（PDPN）的上调在肿瘤侵袭和转移中起重要作用，并且与口腔、皮肤和食管鳞状细胞癌的不良预后显著相关。本研究的目的是调查PDPN在鼻窦鳞状细胞癌（SNSCC）中的表达模式，并评估其作为生存结果预后因素的作用。
本研究纳入了59例SNSCC患者。我们回顾性地从病历中收集了这些患者的临床特征，并获取了相关的福尔马林固定、石蜡包埋组织用于PDPN免疫组织化学染色。此外，分析了PDPN表达与患者临床病理特征和预后的关系。
我们观察到癌细胞和基质癌相关成纤维细胞（CAFs）中PDPN均呈阳性染色。SNSCC患者癌细胞中PDPN的阳性表达与原发肿瘤部位（p = 0.009）和局部复发（p = 0.024）显著相关。此外，PDPN阳性癌细胞患者的总生存期（OS）和无病生存期（DFS）率显著低于PDPN阴性癌细胞患者（均p < 0.05）。多变量分析显示，癌细胞中PDPN表达是OS（p = 0.038）和DFS（p = 0.039）的独立预后因素。
我们的研究结果表明，PDPN过表达可能是SNSCC的独立预后生物标志物和治疗靶点。
