苏丹患者良性和恶性前列腺病变中 Cyclin D1 的免疫组织化学表达。

Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions.

机构信息

Mycetoma Research Center, University of Khartoum, Khartoum, Sudan.

Department of Cytology and Histopathology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.

出版信息

BMC Res Notes. 2020 Jun 17;13(1):295. doi: 10.1186/s13104-020-05138-7.

DOI:10.1186/s13104-020-05138-7

PMID:32552908

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7302005/

Abstract

OBJECTIVES

Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. The study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system.

RESULTS

A total of 153 males' prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

摘要

目的

前列腺癌（PC）是全球常见的癌症。已经开发出几种标志物来区分前列腺增生（BPH）和 PC。本研究旨在确定细胞周期蛋白 D1在 BPH 和 PC 中的表达，是一项描述性回顾性基于医院的研究。该研究于 2016 年 12 月至 2019 年 1 月在苏丹喀土穆州的不同组织病理学实验室进行。福尔马林固定石蜡包埋块被切片并固定在 3-氨丙基三乙氧基硅烷涂覆的载玻片上，孵育细胞周期蛋白 D1的一抗。使用 Gleason 评分系统评估每个切片中细胞周期蛋白 D1的免疫反应性。

结果

本研究共纳入 153 例男性前列腺切片，其中 120 例（78.4%）为 PC，33 例（21.6%）为 BPH。他们的年龄在 45 至 88 岁之间，平均年龄为 66.19±8.599。142 例（92.8%）没有 PC 的家族史，而 11 例（7.2%）患者报告有家族史。Gleason 评分显示共有 81 例（52.9%）患者为高级别，39 例（25.5%）为低级别。118 例（97.5%）有 PC 的患者细胞周期蛋白 D1 检测结果呈阳性，而 BPH 为 3 例（2.5%）。P 值<0.001。细胞周期蛋白 D1染色与高级别 Gleason 评分和神经周围浸润相关，P 值为 0.001。