[急性淋巴细胞白血病患儿微小RNA-146a表达水平、信号转导及转录激活因子1蛋白与临床特征的关系]
[Relationships Between Expression level of MicroRNA-146a, STAT1 Protein and Clinical Characteristics in Children with Acute Lymphoblastic Leukemia].
作者信息
Tan Wen-Min, Huang Fen, Li Guo-Xiong, Wu Jie, Han Feng
机构信息
Department of Clinical Laboratorial Examination,The First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China.
Department of Hematology,The First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Jun;28(3):821-827. doi: 10.19746/j.cnki.issn.1009-2137.2020.03.017.
OBJECTIVE
To analyze the relationships between expression levels of serum microRNA-146a, STAT1 protein and clinical characteristics in children with acute lymphoblastic leukemia (ALL).
METHODS
A total of 102 children diagnosed as ALL in our hospital from June 2014 to June 2016 were enrolled, and were compared by into groups according to clinical characteristics including sex, age, lymphocyte type, disease risk, chemotherapy stage and gene mutation. Fifty healthy children were chosen as control group. The relative expression of microRNA-146a and STAT1 gene was detected by real-time RT-PCR and the relative level of STAT1 protein was detected by Western blot. The difference of microRNA-146a and STAT1 protein levels between clinical factors and laboratory indexs were compared. Followed-up for 3 years, The difference of overall survival (OS) rates between ALL children with different microRNA-146a and STAT1 protein were compared.
RESULTS
The levels of microRNA-146a, STAT1 mRNA and protein in ALL children were significantly higher than those in control group (P＜0.05), but there were no significantly differences in sex, age and lymphocyte type grouping in ALL children (P＞0.05). There were significantly differences in different disease risk, chemotherapy stage and gene mutation groups in ALL children (P＜0.05). Followed-up for 3 years, the OS rate of ALL children with high microRNA-146a and STAT1 protein levels were better than those with low microRNA-146a and STAT1 protein levels (P＜0.05).
CONCLUSION
The up-regulation of microRNA-146a and STAT1 protein may be involved in occurrence and development of ALL, which closely relates to clinical characteristics in ALL children, such as disease risk, chemotherapy stage and gene mutation.
目的
分析急性淋巴细胞白血病（ALL）患儿血清微小RNA-146a、信号转导和转录激活因子1（STAT1）蛋白表达水平与临床特征之间的关系。
方法
选取2014年6月至2016年6月在我院确诊为ALL的102例患儿，根据性别、年龄、淋巴细胞类型、疾病危险度、化疗阶段和基因突变等临床特征进行分组比较。选取50例健康儿童作为对照组。采用实时荧光定量逆转录聚合酶链反应（RT-PCR）检测微小RNA-146a和STAT1基因的相对表达量，采用蛋白质免疫印迹法检测STAT1蛋白的相对水平。比较临床因素和实验室指标之间微小RNA-146a和STAT1蛋白水平的差异。随访3年，比较不同微小RNA-146a和STAT1蛋白水平的ALL患儿总生存（OS）率的差异。
结果
ALL患儿微小RNA-146a、STAT1 mRNA和蛋白水平均显著高于对照组（P＜0.05），但ALL患儿在性别、年龄和淋巴细胞类型分组方面差异无统计学意义（P＞0.05）。ALL患儿在不同疾病危险度、化疗阶段和基因突变组间差异有统计学意义（P＜0.05）。随访3年，微小RNA-146a和STAT1蛋白水平高的ALL患儿OS率优于微小RNA-146a和STAT1蛋白水平低的患儿（P＜0.05）。
结论
微小RNA-146a和STAT1蛋白的上调可能参与ALL的发生发展，与ALL患儿的疾病危险度、化疗阶段和基因突变等临床特征密切相关。
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