Liu Hui, Zhao Jian-Qiang, Ni Zeng-Feng, Liu Yu-Hong, Jiao Jiao
Department of Hematology, The First Affiliated Hospital of Xi'an Medical College, Xi'an 710021, Shaanxi Province, China,E-mail:
Department of Hematology, The First Affiliated Hospital of Xi'an Medical College, Xi'an 710021, Shaanxi Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Jun;28(3):881-885. doi: 10.19746/j.cnki.issn.1009-2137.2020.03.027.
To study the correlation of the expression alteration of Tim-3 with the T cell and B cell dysfunction in peripheral blood of multiple myeloma (MM) patients.
30 patients diagnosed as MM from October 2016 to October 2018 were selected and enrolled in MM group, and 30 healthy persons whose sex and age was matched with the MM patients were selected and enrolled in healthy control group (HC). The blood samples from MM patients and HC were collected, and the peripheral blood mononuclear cells (PBMNC) were separated by density gradient centrifugation, then the serum was kept for further study. The ratios of CD3CD4Tim-3T cells, CD3CD8Tim-3T cells and the CD19+CD20-CD38+B cells were analysed by flow cytometry (FCM)，and the concentration of T cell-related cytokines IFN-γ, TNF-αand B cell-related antibodies IgA, IgM and IgG were measured by ELISA. At the same time, the differences of the ratios of CCD3CD4Tim-3T, CD3CD8Tim-3T cells and plasmablast and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG between the MM patient and HC were estimated, and the correlation of the ratio of CD3CD4Tim-3T, CD3CD8Tim-3T cells with the ratio of plasmablast and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG in MM patients were analyzed.
The ratio of CD3CD4Tim-3T, CD3CD8Tim-3T cells increased in MM patients, while the ratio of CD19+CD20- CD38+B cells and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG decreased in MM patients. And there was a negative correlation of the ratio of CD3CD4Tim-3T cells with CD19+CD20-CD38+B cells and the concentration of IFN-γ, IgA, IgM and IgG in MM patients, while the ratio of CD3CD8Tim-3T cells just negatively correlated with the concentration of TNF-α.
Expression of Tim-3 on CD4 and CD8 cells elevates in the peripheral blood of MM patients, which also correlates with the function suppression of T and B cells.
研究多发性骨髓瘤（MM）患者外周血中Tim-3表达改变与T细胞和B细胞功能障碍的相关性。
选取2016年10月至2018年10月确诊为MM的30例患者纳入MM组，选取30例性别、年龄与MM患者相匹配的健康人纳入健康对照组（HC）。采集MM患者和HC的血样，采用密度梯度离心法分离外周血单个核细胞（PBMNC），然后保存血清用于进一步研究。采用流式细胞术（FCM）分析CD3CD4Tim-3T细胞、CD3CD8Tim-3T细胞及CD19+CD20-CD38+B细胞的比例，采用酶联免疫吸附测定（ELISA）法检测T细胞相关细胞因子IFN-γ、TNF-α及B细胞相关抗体IgA、IgM和IgG的浓度。同时，评估MM患者与HC之间CD3CD4Tim-3T、CD3CD8Tim-3T细胞及浆母细胞比例以及IFN-γ、TNF-α、IgA、IgM和IgG浓度的差异，并分析MM患者中CD3CD4Tim-3T、CD3CD8Tim-3T细胞比例与浆母细胞比例以及IFN-γ、TNF-α、IgA、IgM和IgG浓度的相关性。
MM患者中CD3CD4Tim-3T、CD3CD8Tim-3T细胞比例升高，而CD19+CD20-CD38+B细胞比例以及IFN-γ、TNF-α、IgA、IgM和IgG浓度降低。MM患者中CD3CD4Tim-3T细胞比例与CD19+CD20-CD38+B细胞比例以及IFN-γ、IgA、IgM和IgG浓度呈负相关，而CD3CD8Tim-3T细胞比例仅与TNF-α浓度呈负相关。
MM患者外周血中CD4和CD8细胞上Tim-3的表达升高，这也与T细胞和B细胞的功能抑制相关。
