Department of Infectious Disease and Children's Hospital of Soochow University, Suzhou, China.
Department of Clinical Laboratory, Children's Hospital of Soochow University, Suzhou, China.
Viral Immunol. 2023 Sep;36(7):458-465. doi: 10.1089/vim.2022.0203. Epub 2023 Aug 11.
To investigate the expression and clinical significance of peripheral blood interleukin (IL)-17A, IL-22, T cell immunoglobulin molecule-3 (Tim-3), and galectin-9 (gal-9) in children with infectious mononucleosis (IM) caused by the Epstein-Barr virus (EBV). Peripheral blood of 54 children with IM (case group) was collected and divided into a liver damage group and a non-liver damage group. During the same period, 20 healthy children were in the control group. IL-17A and IL-22 were measured by enzyme-linked immunosorbent assay. Real-time quantitative polymerase chain reaction was used to measure the mRNA expression of Tim-3 and gal-9. Their correlation with clinical indicators was then analyzed. The IL-17A expression level was higher in the case group than in the control group, while Tim-3, gal-9, and IL-22 were lower than those in the control group. Tim-3 was positively correlated with gal-9, but negatively correlated with IL-17A. Tim-3 and gal-9 were positively correlated with CD4/CD8 cells. Conversely, they were negatively correlated with CD3, CD3CD8, white blood cell, lymphocyte (L), alanine transaminase (ALT), aspartate transaminase (AST), glutamyl transpeptidase (GGT), and lactate dehydrogenase (LDH). In the case group, IL-17A was positively correlated with L, GGT, and LDH, but negatively correlated with the natural killer (NK) cell count. IL-17A and IL-22 were positively correlated with CD3, CD3CD8, ALT, and AST, but they were negatively correlated with the ratio of CD4/CD8. In the liver damage group, IL-17A, IL-22, CD3, CD3CD8, immunoglobulin A (IgA), IgG, IgM, L, ALT, AST, GGT, LDH, and -hydroxybutyrate levels were higher than those in the non-liver damage group. However, Tim-3, gal-9, the ratio of CD4/CD8, and NK were lower than those in the non-liver damage group. IL-17A, IL-22, Tim-3, and gal-9 are involved in the immune pathogenesis of IM caused by EBV infection in children, which may be related to immune liver injury.
探讨儿童传染性单核细胞增多症（IM）中由 EBV 引起的外周血白细胞介素（IL）-17A、IL-22、T 细胞免疫球蛋白分子-3（Tim-3）和半乳糖凝集素-9（gal-9）的表达及临床意义。收集 54 例 IM 患儿（病例组）外周血，分为肝损伤组和非肝损伤组。同期，20 例健康儿童为对照组。酶联免疫吸附法检测 IL-17A、IL-22，实时定量聚合酶链反应检测 Tim-3、gal-9 的 mRNA 表达，分析与临床指标的相关性。结果病例组 IL-17A 表达水平高于对照组，Tim-3、gal-9、IL-22 低于对照组。Tim-3 与 gal-9 呈正相关，与 IL-17A 呈负相关。Tim-3、gal-9 与 CD4/CD8 细胞呈正相关，与 CD3、CD3CD8、白细胞、淋巴细胞（L）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、谷氨酰转肽酶（GGT）、乳酸脱氢酶（LDH）呈负相关。病例组 IL-17A 与 L、GGT、LDH 呈正相关，与 NK 细胞计数呈负相关。IL-17A、IL-22 与 CD3、CD3CD8、ALT、AST 呈正相关，与 CD4/CD8 比值呈负相关。肝损伤组 IL-17A、IL-22、CD3、CD3CD8、免疫球蛋白 A（IgA）、IgG、IgM、L、ALT、AST、GGT、LDH、-羟丁酸水平高于非肝损伤组，Tim-3、gal-9、CD4/CD8 比值、NK 细胞低于非肝损伤组。结论 IL-17A、IL-22、Tim-3、gal-9 参与了儿童 EBV 感染所致 IM 的免疫发病机制，可能与免疫性肝损伤有关。
