中国广州细颗粒物中微生物组的分布。
Distribution of Microbiota in Fine Particulate Matter Particles in Guangzhou, China.
机构信息
Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China;Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, Guangdong, China.
Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China;Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, Guangdong, China;Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, Guangdong, China.
出版信息
Biomed Environ Sci. 2020 May 20;33(5):306-314. doi: 10.3967/bes2020.042.
OBJECTIVE
High PM concentration is the main feature of increasing haze in developing states, but information on its microbial composition remains very limited. This study aimed to determine the composition of microbiota in PM in Guangzhou, a city located in the tropics in China.
METHODS
In Guangzhou, from March 5 to 10 , 2016, PM was collected in middle volume air samplers for 23 h daily. The 16S rDNA V4 region of the PM sample extracted DNA was investigated using high-throughput sequence.
RESULTS
Among the Guangzhou samples, , , , , and were the dominant microbiota accounting for more than 90% of the total microbiota, and was the dominant gram-negative bacteria, accounting for 21.30%-23.57%. We examined the difference in bacterial distribution of PM between Beijing and Guangzhou at the genus level; was found in both studies, but was only detected in Guangzhou.
CONCLUSION
In conclusion, the diversity and specificity of microbial components in Guangzhou PM were studied, which may provide a basis for future pathogenicity research in the tropics.
目的
高 PM 浓度是发展中国家霾污染加剧的主要特征，但有关其微生物组成的信息仍然非常有限。本研究旨在确定中国热带城市广州 PM 中的微生物群落组成。
方法
2016 年 3 月 5 日至 10 日，在广州使用中流量空气采样器采集 PM ，每日采集 23 小时。使用高通量测序方法对采集的 PM 样本中提取的 DNA 的 16S rDNA V4 区进行研究。
结果
广州样本中， 、 、 、 、 和 是主要的微生物群，占总微生物群的 90%以上，而 是主要的革兰氏阴性菌，占 21.30%-23.57%。我们在属水平上比较了北京和广州 PM 中细菌分布的差异；两个研究中都发现了 ，但仅在广州检测到 。
结论
综上所述，本研究对广州 PM 中的微生物成分的多样性和特异性进行了研究，这可能为热带地区未来的致病性研究提供依据。
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