Department of Robotics Engineering, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea.
Department of New Biology, DGIST, Daegu, Republic of Korea.
Biosens Bioelectron. 2020 Sep 15;164:112315. doi: 10.1016/j.bios.2020.112315. Epub 2020 May 23.
Zebrafish has been widely used as an experimental animal in many biological processes to study brain functions, neurological disorders and drug toxicity. Electroencephalogram (EEG), which directly measures brain activities, has been used to diagnose and study neurological disorders. Previous studies have recorded EEG signals from adult zebrafish, but the recordings have been only possible from a single individual. In this study, we developed a system to record EEG of multiple adult zebrafish simultaneously. To secure multiple zebrafish in a stable condition for a certain time, perfusion and recording systems were mechanically separated, which allowed effective immobilization of the fish. We recorded EEG signals from pentylenetetrazole (PTZ)-induced seizure models and valproic acid (VPA)-treated models to demonstrate the performance of the developed system in validation of the effect of anti-epileptic drugs. The developed system effectively measured individual EEG signals from more than three zebrafish out of four simultaneously on average. Seizure induction by PTZ and seizure suppression by VPA were successfully detected through EEG recording. The scheme of the EEG recording system developed in this study has a potential for expansion and could be further applied to mass drug screening in a short time.
斑马鱼已被广泛应用于许多生物学过程中的实验动物，以研究大脑功能、神经紊乱和药物毒性。脑电图（EEG）直接测量大脑活动，已被用于诊断和研究神经紊乱。以前的研究已经记录了成年斑马鱼的 EEG 信号，但记录只能来自单个个体。在这项研究中，我们开发了一种同时记录多个成年斑马鱼 EEG 的系统。为了在一定时间内将多个斑马鱼稳定地固定在一个位置，我们将灌流和记录系统机械分离，这允许对鱼进行有效的固定。我们记录了戊四氮（PTZ）诱导的癫痫模型和丙戊酸钠（VPA）处理模型的 EEG 信号，以证明开发的系统在验证抗癫痫药物的效果方面的性能。开发的系统平均能够有效地从四个同时记录的斑马鱼中测量三个以上的个体 EEG 信号。通过 EEG 记录成功检测到了 PTZ 诱导的癫痫发作和 VPA 抑制癫痫发作。本研究中开发的 EEG 记录系统的方案具有扩展潜力，可以进一步应用于短时间内的大规模药物筛选。
