Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Biosens Bioelectron. 2020 Sep 15;164:112329. doi: 10.1016/j.bios.2020.112329. Epub 2020 May 29.
Herein, a novel turn-off fluorescent aptasensor was developed for selective detection of ampicillin (AMP) at picomolar level based on 3,4,9,10-perylenetetracarboxylic acid diimide (PTCDI) as an affordable and low-cost fluorophore. This aptasensor was designed using aptamer, its complementary strand (CS) and gold nanoparticles (AuNPs). The principle of the sensing method is a decrease in the fluorescence intensity of PTCDI in the presence of free CS. Following the addition of AMP, Aptamer/CS-modified AuNPs releases CS and so, the fluorescence intensity of PTCDI is reduced. The designed analytical method indicated a good linear range from 100 pM to 1000 pM and a limit of detection (LOD) of 29.2 pM was obtained. Furthermore, the sensing strategy indicated satisfactory results for the detection of AMP in the spiked human serum samples. By changing the sequences of aptamer and its CS, the presented analytical approach can be easily applied for detection of other antibiotics.
本文报道了一种基于 3,4,9,10-苝四羧酸二酰亚胺（PTCDI）作为一种经济实惠且低成本荧光团的新型关闭型荧光适体传感器，用于在皮摩尔水平上选择性检测氨苄青霉素（AMP）。该适体传感器是使用适体、其互补链（CS）和金纳米粒子（AuNPs）设计的。传感方法的原理是在存在游离 CS 的情况下，PTCDI 的荧光强度降低。加入 AMP 后，Aptamer/CS 修饰的 AuNPs 释放 CS，从而降低了 PTCDI 的荧光强度。所设计的分析方法在 100 pM 至 1000 pM 的范围内呈现出良好的线性关系，检测限（LOD）为 29.2 pM。此外，该传感策略在人血清样品中添加 AMP 的检测中取得了令人满意的结果。通过改变适体及其 CS 的序列，该分析方法可以很容易地应用于其他抗生素的检测。
