从浅海热液喷口（葡萄牙亚速尔群岛）中分离到的一株新的 Alcanivorax sp. VBW004 的基因组特征和铜生物吸附潜力。
Genomic features and copper biosorption potential of a new Alcanivorax sp. VBW004 isolated from the shallow hydrothermal vent (Azores, Portugal).
机构信息
Department of Ecology and Environmental Science, Umeå University, 901 87 Umeå, Sweden; Department of Marine Systems, Tallinn University of Technology, Akadeemia Rd. 15A, 12618 Tallinn, Estonia.
Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India.
出版信息
Genomics. 2020 Sep;112(5):3268-3273. doi: 10.1016/j.ygeno.2020.06.015. Epub 2020 Jun 14.
A new Alcanivorax sp. VBW004 was isolated from a shallow hydrothermal vent in Azores Island, Portugal. In this study, we determined VBW004 was resistant to copper. This strain showed maximum tolerance of copper concentrations up to 600 μg/mL. Based on 16S rRNA gene sequencing and phylogeny revealed that this strain was more closely related to Alcanivorax borkumensis SK2. We sequenced the genome of this strain that consist of 3.8 Mb size with a G + C content of 58.4 %. In addition, digital DNA-DNA hybridizations (dDDH) and the average nucleotide identities (ANI) analysis between Alcanivorax borkumensis SK2 and Alcanivorax jadensis T9 revealed that Alcanivorax sp. VBW004 belongs to new species. Functional annotation revealed that the genome acquired multiple copper resistance encoding genes that could assist VBW004 to respond to high Cu toxicity. Our results from biosorption analysis presumed that the VBW004 is an ecologically important bacterium that could be useful for copper bioremediation.
从葡萄牙亚速尔群岛的一个浅热液喷口分离到一株新的 Alcanivorax sp. VBW004。在本研究中，我们确定 VBW004 对铜具有抗性。该菌株对铜浓度的最大耐受值高达 600μg/mL。基于 16S rRNA 基因测序和系统发育分析，该菌株与 Alcanivorax borkumensis SK2 关系最为密切。我们对该菌株的基因组进行了测序，其大小为 3.8Mb，G+C 含量为 58.4%。此外，通过 DNA-DNA 杂交（dDDH）和 Alcanivorax borkumensis SK2 与 Alcanivorax jadensis T9 之间的平均核苷酸同一性（ANI）分析表明，Alcanivorax sp. VBW004 属于新物种。功能注释表明，该基因组获得了多个铜抗性编码基因，这可能有助于 VBW004 应对高铜毒性。我们的生物吸附分析结果表明，VBW004 是一种具有重要生态学意义的细菌，可用于铜的生物修复。
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