State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, China.
MOE of Key Lab for Coast and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4428-4432. doi: 10.1099/ijs.0.049957-0. Epub 2013 Jul 11.
A taxonomic study was carried out on strain R8-12(T), which was isolated from deep-sea water of the Indian Ocean during the screening of oil-degrading bacteria. The isolate was Gram-stain-negative, oxidase and catalase-positive. Growth was observed at salinities from 0.5 to 15 % (optimum 3 %), at pH from 6-10 (optimum 7-8) and at temperatures from 10 to 42 °C (optimum 28 °C). On the basis of 16S rRNA gene sequence similarity, strain R8-12(T) was shown to belong to the genus Alcanivorax and to be related to Alcanivorax venustensis DSM 13974(T) (97.2 %), A. dieselolei B-5(T) (95.0 %), A. balearicus MACL04(T) (94.6 %), A. hongdengensis A-11-3(T) (94.3 %), A. jadensis T9(T) (93.8 %), A. borkumensis SK2(T) (93.7 %) and A. pacificus W11-5(T) (93.7 %). The gyrB sequence similarities between R8-12(T) and other species of the genus Alcanivorax ranged from 77.9 % to 86.9 %. The major fatty acids were C16 : 0 (31.8 %), C18 : 1ω7c (20.3 %), C19 : 0ω8c cyclo (15.8 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (8.9 %). The polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two aminolipids (AL1-AL2) and two phospholipids (PL1-PL2). Three alkane hydroxylase (alkB) genes were identified in the genome. The G+C content of the chromosomal DNA was 66.1 mol%. DNA-DNA hybridization showed that strain R8-12(T) and A. venustensis DSM 13974(T) had a DNA-DNA relatedness of 63±3 %. According to its phenotypic features and fatty acid composition as well as the 16S rRNA and gyrB gene sequences, the novel strain represents a member of the genus Alcanivorax, but could be easily distinguished from all other known species of the genus Alcanivorax described to date. The name Alcanivorax marinus sp. nov. is proposed, with the type strain R8-12(T) ( = MCCC 1A00382(T) = LMG 24621(T) = CCTCC AB 208234(T)).
对 R8-12(T) 菌株进行了分类学研究，该菌株是在筛选石油降解菌时从印度洋深海海水中分离出来的。该分离株革兰氏阴性，氧化酶和过氧化氢酶阳性。在盐度为 0.5%至 15%（最佳 3%）、pH 值为 6-10（最佳 7-8）和温度为 10-42°C（最佳 28°C）的条件下可以观察到生长。基于 16S rRNA 基因序列相似性，菌株 R8-12(T) 属于 Alcanivorax 属，与 Alcanivorax venustensis DSM 13974(T)（97.2%）、A. dieselolei B-5(T)（95.0%）、A. balearicus MACL04(T)（94.6%）、A. hongdengensis A-11-3(T)（94.3%）、A. jadensis T9(T)（93.8%）、A. borkumensis SK2(T)（93.7%）和 A. pacificus W11-5(T)（93.7%）最为接近。R8-12(T)与 Alcanivorax 属其他种的 gyrB 序列相似性在 77.9%至 86.9%之间。主要脂肪酸为 C16:0（31.8%）、C18:1ω7c（20.3%）、C19:0ω8c 环（15.8%）和特征性脂肪酸 3（C16:1ω6c 和/或 C16:1ω7c）（8.9%）。极性脂类包括磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、两种胺脂（AL1-AL2）和两种磷脂（PL1-PL2）。在基因组中鉴定出了三种烷烃羟化酶（alkB）基因。染色体 DNA 的 G+C 含量为 66.1mol%。DNA-DNA 杂交显示，菌株 R8-12(T) 和 A. venustensis DSM 13974(T) 的 DNA-DNA 相关性为 63±3%。根据其表型特征、脂肪酸组成以及 16S rRNA 和 gyrB 基因序列，该新菌株代表 Alcanivorax 属的一个成员，但与迄今为止描述的所有其他已知属 Alcanivorax 种均易于区分。建议使用新名称 Alcanivorax marinus sp. nov.，其模式菌株为 R8-12(T)（=MCCC 1A00382(T)=LMG 24621(T)=CCTCC AB 208234(T)）。
