Ye C Q, Folkers K, Tamagawa H, Pfeiffer C
Institute for Biomedical Research, University of Texas, Austin.
Biofactors. 1988 Dec;1(4):303-6.
Two situations required a modified determination of coenzyme Q10 (CoQ10) in human blood and organ tissue. Blood from patients with AIDS and cancer raised apprehensions about safety to an analyst, and the number of specimens for analysis is increasing enormously. A modified determination replaces silica gel-TLC with disposable Florisil columns, and steps were simplified to allow more analyses per unit time. Data from the modified determination are quantitatively compatible with data from older and tedious procedures. This determination was used for blood from 36 diverse patients with allergies. The mean CoQ10 blood level of these patients is not different from the mean level of so-called normal individuals, but approximately 40% (14/36) of these allergic patients had levels up to 0.65 micrograms/ml, which is the level of dying class IV cardiac patients. The biosynthesis of CoQ10 in human tissues is a complex process that requires several vitamins and micronutrients, so that countless vitamin-unsupplemented Americans may be deficient in CoQ10. The relationship of allergies to autoimmune mechanisms and immunity, and the established relationship of CoQ10 to immune states, may be a rationale for therapeutic trials of administering CoQ10 to patients with allergies who have low CoQ10 blood levels and are very likely deficient.
有两种情况需要对人体血液和器官组织中的辅酶Q10（CoQ10）进行改进测定。艾滋病患者和癌症患者的血液让分析人员对安全性产生担忧，且分析样本数量正大幅增加。改进后的测定方法用一次性弗罗里硅土柱取代了硅胶薄层层析法，并简化了步骤，以便在单位时间内进行更多分析。改进测定得到的数据在定量方面与旧的繁琐程序得到的数据相符。该测定方法用于36名不同过敏患者的血液。这些患者的CoQ10血液平均水平与所谓正常个体的平均水平并无差异，但约40%（14/36）的这些过敏患者的CoQ10水平高达0.65微克/毫升，这是IV级濒死心脏病患者的水平。人体组织中CoQ10的生物合成是一个复杂过程，需要多种维生素和微量营养素，因此无数未补充维生素的美国人可能缺乏CoQ10。过敏与自身免疫机制和免疫的关系，以及CoQ10与免疫状态已确立的关系，可能是对CoQ10血液水平低且很可能缺乏的过敏患者进行CoQ10治疗试验的一个理由。
