Palumbo A, Turco G L, Brossa C, D'Alberto M, Ghezzo F, Pegoraro L
Boll Soc Ital Biol Sper. 1980 Jul 15;56(13):1350-4.
The specific insulin binding activity of human promyelocitic HL 60 cell line during the myeloid and macrophagic differentiation induced by chemical compounds was investigated. Dimethyl sulfoxide (DMSO) and retinoic acid myeloid induced differentiation in HHL 60 cells was accompanied by a marked decrease of insulin receptors. In K 562 cell line, where DMS O has no effect on differentiation, the number of insulin receptors was only slightly affected. 12-0-tetradodecanoil phorbol 13-acetate (TPA) induced macrophagic differentiation of HL 60 cell line was also accompanied by a decrease of insulin binding activity. Our results support the hypothesis that during the process of terminal differentiation a decrease of insulin receptors occurs.
研究了化合物诱导人早幼粒细胞HL 60细胞系髓系和巨噬系分化过程中的特异性胰岛素结合活性。二甲基亚砜（DMSO）和维甲酸诱导HHL 60细胞系的髓系分化，同时胰岛素受体显著减少。在K 562细胞系中，DMSO对分化无影响，胰岛素受体数量仅略有影响。12 - O -十四烷酰佛波醇13 -乙酸酯（TPA）诱导HL 60细胞系的巨噬系分化也伴随着胰岛素结合活性的降低。我们的结果支持这样的假设：在终末分化过程中会出现胰岛素受体减少的情况。
