α-神经毒素在人乙酰胆碱受体α亚基细胞外部分的结合区域。
The regions of alpha-neurotoxin binding on the extracellular part of the alpha-subunit of human acetylcholine receptor.
作者信息
Mulac-Jericevic B, Manshouri T, Yokoi T, Atassi M Z
机构信息
Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
出版信息
J Protein Chem. 1988 Apr;7(2):173-7. doi: 10.1007/BF01025247.
A set of 18 synthetic uniform overlapping peptides spanning the entire extracellular part (residues 1-210) of the alpha-subunit of human acetylcholine receptor were studied for their binding activity of 125I-labeled alpha-bungarotoxin and cobratoxin. A major toxin-binding region was found to reside within peptide alpha 122-138. In addition, low-binding activities were obtained with peptides alpha 34-49 and alpha 194-210. It is concluded that the region within residues alpha 122-138 constitutes a universal major toxin-binding region for acetylcholine receptor of various species.
研究了一组18个合成的均匀重叠肽，这些肽跨越人乙酰胆碱受体α亚基的整个细胞外部分（第1至210位氨基酸残基），检测它们与125I标记的α-银环蛇毒素和眼镜蛇毒素的结合活性。发现一个主要的毒素结合区域位于肽α122 - 138内。此外，肽α34 - 49和α194 - 210也表现出低结合活性。得出的结论是，α122 - 138位氨基酸残基区域构成了各种物种乙酰胆碱受体的一个通用主要毒素结合区域。
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