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一种用于阐明蛋白质抗原结构的新颖且全面的合成方法。人血红蛋白α链完整抗原谱的测定。

A novel and comprehensive synthetic approach for the elucidation of protein antigenic structures. Determination of the full antigenic profile of the alpha-chain of human haemoglobin.

作者信息

Kazim A L, Atassi M Z

出版信息

Biochem J. 1980 Oct 1;191(1):261-4. doi: 10.1042/bj1910261.

DOI:10.1042/bj1910261
PMID:6162459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162207/
Abstract

A comprehensive synthetic approach for the determination of continuous antigenic sites of proteins is presented. This approach consists of the synthesis of a series of consecutive overlapping peptides that, together, systematically represent the entire primary structure of the protein under study. Its application to the alpha-chain of human haemoglobin afforded, for the first time, a full profile of immunochemically active alpha-chain peptides and enabled the localization of all the major continuous antigenic sites of this haemoglobin subunit.

摘要

本文提出了一种用于确定蛋白质连续抗原位点的综合合成方法。该方法包括合成一系列连续重叠的肽段，这些肽段共同系统地代表了所研究蛋白质的整个一级结构。将其应用于人血红蛋白的α链，首次得到了免疫化学活性α链肽段的完整图谱，并确定了该血红蛋白亚基所有主要连续抗原位点的位置。

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1
A novel and comprehensive synthetic approach for the elucidation of protein antigenic structures. Determination of the full antigenic profile of the alpha-chain of human haemoglobin.一种用于阐明蛋白质抗原结构的新颖且全面的合成方法。人血红蛋白α链完整抗原谱的测定。
Biochem J. 1980 Oct 1;191(1):261-4. doi: 10.1042/bj1910261.
2
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J Protein Chem. 1993 Aug;12(4):403-12. doi: 10.1007/BF01025040.
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本文引用的文献

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Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
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Immunochemistry of sperm-whale myoglobin. XVI. Accurate delineation of the single region in sequence 1-55 by immunochemical studies of synthetic peptides. Some conclusions concerning antigenic structures of proteins.抹香鲸肌红蛋白的免疫化学。十六。通过合成肽的免疫化学研究精确描绘序列1 - 55中的单个区域。关于蛋白质抗原结构的一些结论。
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Immunochemistry of sperm-whale myoglobin. 18. Accurate delineation of the single reactive region in sequence 120-153 by study of synthetic peptides.抹香鲸肌红蛋白的免疫化学。18. 通过合成肽研究精确描绘序列120 - 153中的单个反应区域。
Biochim Biophys Acta. 1973 Dec 6;328(2):289-302.
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Periodate oxidation of sperm-whale myoglobin and the role of the methionine residues in the antigen-antibody reaction.抹香鲸肌红蛋白的高碘酸盐氧化作用以及甲硫氨酸残基在抗原 - 抗体反应中的作用。
Biochem J. 1967 Feb;102(2):478-87. doi: 10.1042/bj1020478.
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Use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites.免疫吸附剂在研究抗体与抹香鲸肌红蛋白及其合成抗原位点结合中的应用。
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Precise determination of the entire antigenic structure of lysozyme: molecular features of protein antigenic structures and potential of "surface-simulation" synthesis--a powerful new concept for protein binding sites.溶菌酶完整抗原结构的精确测定：蛋白质抗原结构的分子特征及“表面模拟”合成的潜力——蛋白质结合位点的一个强大新概念
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