腹泻和健康犊牛体内大肠杆菌的毒力基因
Virulence genes of Escherichia coli in diarrheic and healthy calves.
作者信息
Umpiérrez Ana, Ernst Déborah, Fernández Magalí, Oliver Martín, Casaux María L, Caffarena Rubén D, Schild Carlos, Giannitti Federico, Fraga Martín, Zunino Pablo
机构信息
Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
出版信息
Rev Argent Microbiol. 2021 Jan-Mar;53(1):34-38. doi: 10.1016/j.ram.2020.04.004. Epub 2020 Jun 16.
Escherichia coli ETEC, EPEC, NTEC and STEC/EHEC pathotypes are often isolated from bovine feces. The objective of this study was to detect 21 E. coli virulence genes in feces from 252 dairy calves in Uruguay (149 with neonatal diarrhea - NCD - and 103 asymptomatic). Genes iucD, f17A, afa8E, papC, clpG and f17G(II) were the most prevalent (81.3%; 48.4%; 37.3%; 35.7%; 34.1%; 31.3%, respectively). Genes eae, stx1and stx2 were poorly represented; 13/252 animals harbored one or a combination of these genes. The prevalence of the cnf gene was 4.4%, while that of cdt-IV and cdt-III genes was 24.2% and 12.7% respectively. This study reports updated data about the virulence profiles of E. coli in dairy calves in Uruguay. A large number of adhesins and toxin genes were detected. Our results demonstrate that E. coli from bovine feces has diarrheagenic and extraintestinal profiles although other NCD risks factors may contribute to the disease outcome.
大肠埃希菌的肠毒素型大肠杆菌（ETEC）、肠致病性大肠杆菌（EPEC）、非典型肠毒素型大肠杆菌（NTEC）和肠出血性大肠杆菌（STEC/EHEC）致病型常从牛粪便中分离出来。本研究的目的是检测乌拉圭252头奶牛犊牛（149头患有新生儿腹泻 - NCD - 和103头无症状）粪便中的21种大肠杆菌毒力基因。基因iucD、f17A、afa8E、papC、clpG和f17G(II)最为常见（分别为81.3%；48.4%；37.3%；35.7%；34.1%；31.3%）。基因eae、stx1和stx2的比例较低；252只动物中有13只携带这些基因中的一种或几种组合。cnf基因的流行率为4.4%，而cdt-IV和cdt-III基因的流行率分别为24.2%和12.7%。本研究报告了乌拉圭奶牛犊牛大肠杆菌毒力谱的最新数据。检测到大量粘附素和毒素基因。我们的结果表明，牛粪便中的大肠杆菌具有致腹泻和肠外致病谱，尽管其他NCD风险因素可能会影响疾病结果。
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