Xu Yeling, Chen Weihao, Yang Huiguo, Song Zhenghai, Wang Yeqing, Su Rui, Mwacharo Joram M, Lv Xiaoyang, Sun Wei
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Institute of Animal Husbandry, Xinjiang Academy of Animal Sciences, Urumqi 830013, China.
Vet Sci. 2024 May 8;11(5):206. doi: 10.3390/vetsci11050206.
Diarrhea is the most common issue in sheep farms, typically due to pathogenic () infections, such as F17. microRNA, a primary type of non-coding RNA, has been shown to be involved in diarrhea caused by pathogenic . To elucidate the profound mechanisms of miRNA in F17 infections, methods such as F17 adhesion assay, colony counting assay, relative quantification of bacterial fimbriae gene expression, indirect immune fluorescence (IF), Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), Western blotting (WB), and scratch assay were conducted to investigate the effect of miR-329b-5p overexpression/knock-down on F17 susceptibility of sheep intestinal epithelial cells (IECs). The findings indicated that miR-329b-5p enhances the F17 resistance of sheep IECs to F17 by promoting adhesion between F17 and IEC, as well as IEC proliferation and migration. In summary, miR-329b-5p plays a crucial role in the defense of sheep IECs against F17 infection, providing valuable insights into its mechanism of action.
腹泻是养羊场最常见的问题，通常是由致病性（）感染引起的，如F17。微小RNA是一种主要的非编码RNA，已被证明与致病性引起的腹泻有关。为了阐明微小RNA在F17感染中的深层机制，进行了F17黏附试验、菌落计数试验、细菌菌毛基因表达的相对定量、间接免疫荧光（IF）、细胞计数试剂盒-8（CCK-8）、5-乙炔基-2'-脱氧尿苷（EdU）、蛋白质免疫印迹法（WB）和划痕试验等方法，以研究miR-329b-5p过表达/敲低对绵羊肠上皮细胞（IECs）对F17易感性的影响。研究结果表明，miR-329b-5p通过促进F17与IEC之间的黏附以及IEC的增殖和迁移，增强了绵羊IECs对F17的抗性。总之，miR-329b-5p在绵羊IECs抵御F17感染的防御中起关键作用，为其作用机制提供了有价值的见解。
