Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bang Khun Thain, Bangkok 10150, Thailand.
Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bang Khun Thain, Bangkok 10150, Thailand.
Int J Biol Macromol. 2020 Oct 15;161:1021-1028. doi: 10.1016/j.ijbiomac.2020.06.104. Epub 2020 Jun 15.
Caulerpa is a marine macroalgae and is rich in polysaccharides, which have the potential for immunostimulatory and anticoagulant activity. The objective of this work was to increase the value of C. lentillifera waste by polysaccharide extraction. A polysaccharide yield of about 25% of dry weight was obtained under the following optimized conditions: two-stage extraction (60 min/stage) using a ratio of 1:15 (w/v) at 90 °C, and 2× precipitation by the final concentration of 75% ethanol. The polysaccharide extracts contained a non-reducing sugar that accounted for 44% of weight extracts as a major sugar and consisted of four neutral sugars: mannose (33.3%), galactose (31.9%,), glucose (27.0%) and xylose (7.6%). In addition, it contained sulfate, which is approximately 8.37% of weight extracts and had a phenolic content of around 1.27 mg GAE/g sample. Moreover, it demonstrated α-glucosidase inhibitory activity with an IC value of 13.59 mg/mL. This result suggests that the polysaccharide extracts could potentially be used for preventing diabetes disease. The economic analysis also showed an economic feasibility for producing polysaccharide extracts from C. lentillifera waste. This is an alternative for farmers in order to increase the value of C. lentillifera waste.
石莼是一种海洋大型藻类，富含多糖，具有免疫刺激和抗凝活性。本工作旨在通过多糖提取提高石莼废物的附加值。在以下优化条件下，多糖得率约为干重的 25%：90°C 下，1:15（w/v）的比例进行两阶段提取（60 分钟/阶段），最终浓度为 75%乙醇进行 2 次沉淀。多糖提取物含有一种非还原糖，占提取物重量的 44%，主要由四种中性糖组成：甘露糖（33.3%）、半乳糖（31.9%）、葡萄糖（27.0%）和木糖（7.6%）。此外，它还含有硫酸盐，约占提取物重量的 8.37%，酚类含量约为 1.27 mg GAE/g 样品。此外，它还表现出α-葡萄糖苷酶抑制活性，IC 值为 13.59 mg/mL。这一结果表明，多糖提取物有望用于预防糖尿病。经济分析还表明，从石莼废物中生产多糖提取物具有经济可行性。这是农民增加石莼废物附加值的一种选择。
