一种来自……的新型多糖可诱导巨噬细胞RAW264.7发生免疫调节的分子变化。 （原文中“from”后面内容缺失）
A new polysaccharide from induces molecular shifts of immunomodulation on macrophages RAW264.7.
作者信息
Wu Yulin, Liu Jun, Hao Huili, Hu Lianmei, Zhang Xiaoyong, Luo Lianxiang, Zeng Jincheng, Zhang Wei, Nam Wong Io, Huang Riming
机构信息
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Laboratory of Pathogenic Biology, The Marine Biomedical Research Institute, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
出版信息
Food Chem X. 2022 Apr 19;14:100313. doi: 10.1016/j.fochx.2022.100313. eCollection 2022 Jun 30.
Investigation on polysaccharides led to the finding of a new polysaccharide (CCP). The basic components of CCP were the total sugar (59.18% ± 0.57%), the uronic acids (36.75% ± 0.28%) and the sulfate (42.50% ± 0.42%), in total content. The physicochemical analysis revealed that CCP was a heteropolysaccharide with a molecular weight of 321.6 KDa, and composed of arabinose, fucose, glucose, mannose, galactose, xylose, fructose, ribose, glucuronic acid and galacturonic acid. The immunomodulatory assay showed that CCP played an important role in activating cell viability, the nitric oxide product and cytokines (IL-6 and TNF-α) secretion. Furthermore, the transcript-metabolic analysis displayed a total of 7692 differentially expressed genes (DEGs) and 95 differentially accumulated metabolites (DAMs), and revealed that CCP may play an immunomodulatory effect by activating NF-κB signaling pathway and arachidonic acid metabolism pathway. These findings will provide a basic understanding to further investigation of polysaccharides.
对多糖的研究发现了一种新的多糖（CCP）。CCP的基本成分包括总糖（59.18%±0.57%）、糖醛酸（36.75%±0.28%）和硫酸盐（42.50%±0.42%），这些成分的总含量。物理化学分析表明，CCP是一种分子量为321.6 kDa的杂多糖，由阿拉伯糖、岩藻糖、葡萄糖、甘露糖、半乳糖、木糖、果糖、核糖、葡萄糖醛酸和半乳糖醛酸组成。免疫调节试验表明，CCP在激活细胞活力、一氧化氮生成和细胞因子（IL-6和TNF-α）分泌方面发挥重要作用。此外，转录组-代谢分析共显示7692个差异表达基因（DEGs）和95个差异积累代谢物（DAMs），并表明CCP可能通过激活NF-κB信号通路和花生四烯酸代谢通路发挥免疫调节作用。这些发现将为进一步研究多糖提供基础认识。
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