School of Ocean Sciences, China University of Geosciences (Beijing), Beijing, 100083, China.
School of Ocean Sciences, China University of Geosciences (Beijing), Beijing, 100083, China; Beijing Key Laboratory of Water Resources and Environmental Engineering, China University of Geosciences (Beijing), Beijing, 100083, China.
Chemosphere. 2020 Nov;258:127375. doi: 10.1016/j.chemosphere.2020.127375. Epub 2020 Jun 12.
Petroleum has contaminated microbial habitats in some parts of permafrost. The microbial community has probably undergone great changes due to the differential sensitivity of bacteria to petroleum contamination, making the seasonally frozen ground ecosystem even more fragile. In this study, we analyzed the microbial community structure and function at different soil depths and petroleum contaminant levels, and studied their relationship with environmental factors through correlation analysis, the random forest algorithm and co-occurrence network analysis. We found that microbial community composition and function mainly varied in response to concentrations of petroleum and sulfates in the environment. The microbial community was divided into six modules as functional groups. Among them, sulfate-reducing bacteria and sulfite-oxidizing bacteria play important roles in module0 and module4, respectively, which were possibly responsible for the degradation of petroleum in permafrost zone. The microbial ability to degrade petroleum decreased and glycan metabolism decreased and then increased through the temperature rise-fall process as a result of microbial stress tolerance mechanisms to pollution and temperature changes. The impact on microbial community structure and function, as well as the responses to petroleum pollution and temperature changes, are revealed in this study.
石油已污染了多年冻土区部分地区的微生物栖息地。由于细菌对石油污染的敏感性存在差异，微生物群落可能已经发生了巨大变化，使季节性冻土生态系统更加脆弱。在这项研究中，我们分析了不同土壤深度和石油污染水平下的微生物群落结构和功能，并通过相关性分析、随机森林算法和共生网络分析研究了它们与环境因素的关系。我们发现，微生物群落组成和功能主要响应环境中石油和硫酸盐的浓度而变化。微生物群落被分为六个模块作为功能群。其中，硫酸盐还原菌和亚硫酸盐氧化菌分别在模块 0 和模块 4 中发挥重要作用，它们可能负责降解冻土带中的石油。随着微生物对污染和温度变化的应激耐受机制的作用，微生物降解石油的能力下降，聚糖代谢先下降后增加。本研究揭示了微生物群落结构和功能以及对石油污染和温度变化的响应。
