高胆固醇饮食促进大鼠精氨酸酶和胆碱能酶系统功能障碍：咖啡酸和绿原酸的改善作用。

High cholesterol diet promotes dysfunction of arginase and cholinergic enzymatic system in rats: ameliorative role of caffeic and chlorogenic acids.

机构信息

Department of Biochemistry, Federal University of Technology, Functional Foods, Nutraceuticals and Phytomedicine Unit, Akure, P.M.B. 704, Akure, 340001, Nigeria.

Department of Biochemistry, Federal University of Technology, Functional Foods, Nutraceuticals and Phytomedicine Unit, Akure, P.M.B. 704, Akure, Nigeria.

出版信息

J Complement Integr Med. 2020 Jun 18;18(1):67-74. doi: 10.1515/jcim-2019-0271.

DOI:10.1515/jcim-2019-0271

PMID:32554834

Abstract

BACKGROUND

Dietary phenolic compounds intake have been reported to have an inverse relationship to the prevalence of hypercholesterolemia. The objective of this study is to determine the effect of caffeic acid (CFA) and chlorogenic acid (CGA) on rats fed with high cholesterol diet (HCD).

METHODS

Experimental animals were fed with high cholesterol diet (HCD) for a period of 21 days while simvastatin (0.2 mg/kg BWT), CFA and CGA (10 and 15 mg/kg BWT) were administered daily.

RESULTS

Activity of acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and arginase were significantly (P<0.05) higher in the rats fed with HCD alone. Also, level of malondiadehyde equivalent compounds (MDA) was significantly (P<0.05) elevated in hypercholesterolemic rats. Nevertheless, treatment with simvastatin, CFA and CGA normalized altered AChE, BChE and arginase activities as well as improved antioxidant status in hypercholesterolemic rats.

CONCLUSION

CFA and CGA could offer protective role in hypercholeseterolemic rats via their antioxidant potentials as well as restoring altered activity of acetylcholinesterase, butrylcholinesterase and arginase. Based on our findings chlorogenic acid exhibits better attribute.

摘要

背景

膳食酚类化合物的摄入与高胆固醇血症的患病率呈负相关。本研究的目的是确定咖啡酸（CFA）和绿原酸（CGA）对高胆固醇饮食（HCD）喂养大鼠的影响。

方法

实验动物连续 21 天给予高胆固醇饮食（HCD），同时给予辛伐他汀（0.2mg/kg BWT）、CFA 和 CGA（10 和 15mg/kg BWT）。

结果

单独给予 HCD 的大鼠乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和精氨酸酶活性显著升高（P<0.05）。此外，高胆固醇血症大鼠的丙二醛当量化合物（MDA）水平显著升高（P<0.05）。然而，辛伐他汀、CFA 和 CGA 治疗可使高胆固醇血症大鼠的 AChE、BChE 和精氨酸酶活性恢复正常，并改善抗氧化状态。