长链非编码 RNA MORT 通过下调 microRNA-146a-5p 调控膀胱癌行为。

lncRNA MORT Regulates Bladder Cancer Behaviors by Downregulating MicroRNA-146a-5p.

机构信息

Department of Urology, The Affiliated Huaian No. 1 Hospital of Nanjing Medical University, Huaian City, China,

Department of Urology, The Affiliated Huaian No. 1 Hospital of Nanjing Medical University, Huaian City, China.

出版信息

Nephron. 2020;144(7):351-357. doi: 10.1159/000506291. Epub 2020 Jun 17.

DOI:10.1159/000506291

PMID:32554962

Abstract

BACKGROUND

Long noncoding RNAs (lncRNAs) are involved in the progression of various cancers. lncRNA MORT is downregulated in bladder cancer, while its function in this disease is unknown.

METHODS

lncRNA MORT and miR-146a-5p expression in 56 bladder cancer patients was detected by RT-qPCR. Correlations between MORT and miR-146a-5p were analyzed by Pearson's correlation coefficient. CCK-8 and flow transwell assays were applied to examine the behavioral changes in HT-1197 and HT-1376.

RESULTS

We found that miR-146a-5p was upregulated, while lncRNA MORT was downregulated in bladder cancer. miR-146a-5p and MORT were inversely and significantly correlated in tumor tissues. Overexpression of miR-146a-5p promoted, while overexpression of lncRNA MORT inhibited the invasion, migration, and proliferation of cells of bladder cancer cell lines. In addition, overexpression of lncRNA MORT inhibited miR-146a-5p; miR-146a-5p overexpression failed to significantly affect lncRNA MORT expression but attenuated its inhibitory effects on cancer cell behaviors.

CONCLUSION

lncRNA MORT may regulate bladder cancer cell behaviors by downregulating miR-146a-5p.

摘要

背景

长链非编码 RNA（lncRNA）参与多种癌症的进展。lncRNA MORT 在膀胱癌中下调，但其在该疾病中的功能尚不清楚。

方法

通过 RT-qPCR 检测 56 例膀胱癌患者的 lncRNA MORT 和 miR-146a-5p 表达。通过 Pearson 相关系数分析 MORT 与 miR-146a-5p 之间的相关性。应用 CCK-8 和流式细胞术检测 HT-1197 和 HT-1376 的行为变化。

结果

我们发现 miR-146a-5p 在膀胱癌中上调，而 lncRNA MORT 下调。肿瘤组织中 miR-146a-5p 和 MORT 呈负相关且显著相关。miR-146a-5p 的过表达促进了细胞侵袭、迁移和增殖，而过表达 lncRNA MORT 则抑制了膀胱癌细胞系的这些行为。此外，lncRNA MORT 过表达抑制了 miR-146a-5p；miR-146a-5p 的过表达并未显著影响 lncRNA MORT 的表达，但减弱了其对癌细胞行为的抑制作用。