Departments of Pathology.
Family, Population, and Preventive Medicine.
Appl Immunohistochem Mol Morphol. 2021 Feb 1;29(2):144-151. doi: 10.1097/PAI.0000000000000862.
The major roles of keratin 17 (K17) as a prognostic biomarker have been highlighted in a range of human malignancies. However, its relevance to esophageal squamous cell carcinoma (ESCC) remains unexplored. In this study, the relationship between K17 expression and clinicopathologic parameters and survival were determined by RNA sequencing (RNA-Seq) in 90 ESCCs and by immunohistochemistry (IHC) in 68 ESCCs. K17 expression was significantly higher in ESCC than in paired normal tissues at both the messenger RNA and protein levels. K17 messenger RNA and staining by IHC were significantly correlated with aggressive characteristics, including advanced clinical stage, invasion depth, and lymph node metastases; and were predictive of poor prognosis in advanced disease patients. Furthermore, K17 expression was detected by IHC in high-grade premalignant lesions of the esophageal mucosa, suggesting that K17 could also be a biomarker of dysplasia of the esophageal mucosa. Overall, this study established that K17 is a negative prognostic biomarker for the most common subtype of esophageal cancer.
角蛋白 17（K17）作为一种预后生物标志物的主要作用已在多种人类恶性肿瘤中得到强调。然而，其与食管鳞状细胞癌（ESCC）的相关性尚未得到探索。在这项研究中，通过 90 例 ESCC 的 RNA 测序（RNA-Seq）和 68 例 ESCC 的免疫组织化学（IHC）确定了 K17 表达与临床病理参数和生存之间的关系。在信使 RNA 和蛋白质水平上，K17 在 ESCC 中的表达均明显高于配对的正常组织。K17 信使 RNA 和 IHC 染色与侵袭性特征显著相关，包括临床晚期、浸润深度和淋巴结转移；并且在晚期疾病患者中具有不良预后的预测性。此外，IHC 在食管黏膜高级别癌前病变中检测到 K17 表达，表明 K17 也可能是食管黏膜发育不良的生物标志物。总的来说，这项研究确立了 K17 是最常见的食管癌症亚型的负预后生物标志物。
