Department of Microbiology with Parasitology and Immunology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia.
Institute of Public Health of Vojvodina, Futoška 121, Novi Sad, Serbia.
J Mol Histol. 2020 Aug;51(4):367-374. doi: 10.1007/s10735-020-09886-5. Epub 2020 Jun 15.
Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups-control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group.
慢性应激被认为是许多疾病发病的重要因素。已经开发出许多方法来研究慢性应激的影响。本研究的目的是通过条件性致瘤细胞系 BHK-21/C13 来挑战，展示用于分析慢性束缚应激诱导的免疫抑制的新实验模型。20 只雄性 NMRI 小鼠被随机分为对照组和实验组。每组小鼠均皮下接种 BHK-21/C13 细胞。实验组应激持续 20 天。实验结束后，切除肿瘤块，并用组织学和免疫组织化学技术进行分析。我们发现两组之间在肿瘤表达和肿瘤体积方面存在统计学显著差异（p=0.034），以及在 Ki67、细胞色素 C 和基质金属蛋白酶 9 的免疫阳性率方面存在统计学显著差异（p=0.0061）。实验组未发现免疫浸润，而对照组在肿瘤侵袭前沿存在炎症浸润。
