病毒诱导的甜罗勒（Ocimum basilicum）基因沉默。

Virus-Induced Gene Silencing in Sweet Basil (Ocimum basilicum).

机构信息

Biotechnology Division, Council of Scientific and Industrial Research-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.

Metabolic Biology Department,, John Innes Centre, Norwich, United Kingdom.

出版信息

Methods Mol Biol. 2020;2172:123-138. doi: 10.1007/978-1-0716-0751-0_10.

DOI:10.1007/978-1-0716-0751-0_10

PMID:32557366

Abstract

Virus-induced gene silencing (VIGS) is a powerful reverse genetic tool for rapid functional analysis of plant genes. Over the last decade, VIGS has been widely used for conducting rapid gene knockdown experiment in plants and played a crucial role in advancing applied and basic research in plant science. VIGS was studied extensively in model plants Arabidopsis and tobacco. Moreover, several non-model plants such as Papaver (Hileman et al., Plant J 44:334-341, 2005), Aquilegia (Gould and Kramer, Plant Methods 3:6, 2007), Catharanthus (Liscombe and O'Connor, Phytochemistry 72:1969-1977, 2011), Withania (Singh et al., Plant Biol J 13:1287-1299, 2015), and Ocimum (Misra et al., New Phytol 214:706-720, 2017) were also successfully explored. We have recently developed a robust protocol for VIGS in sweet basil (Ocimum basilicum). Sweet basil, a popular medicinal/aromatic herb, is being studied for the diversity of specialized metabolites produced in it.

摘要

病毒诱导的基因沉默 (VIGS) 是一种强大的反向遗传学工具，可用于快速分析植物基因的功能。在过去的十年中，VIGS 已被广泛用于在植物中进行快速基因敲低实验，并在推进植物科学的应用和基础研究方面发挥了关键作用。VIGS 在模式植物拟南芥和烟草中得到了广泛研究。此外，一些非模式植物，如罂粟（Hileman 等人，Plant J 44:334-341, 2005）、金鱼草（Gould 和 Kramer，Plant Methods 3:6, 2007）、长春花（Liscombe 和 O'Connor，Phytochemistry 72:1969-1977, 2011）、龙舌兰（Singh 等人，Plant Biol J 13:1287-1299, 2015）和罗勒（Ocimum basilicum）（Misra 等人，New Phytol 214:706-720, 2017）也被成功探索。我们最近开发了一种在甜罗勒（Ocimum basilicum）中进行 VIGS 的稳健方案。甜罗勒是一种受欢迎的药用/芳香草药，因其产生的多种特殊代谢物而受到研究。