Navet Natasha, Tian Miaoying
Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, USA.
Bio Protoc. 2020 Nov 20;10(22):e3828. doi: 10.21769/BioProtoc.3828.
Sweet basil () is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding and deploy effective disease management strategies, and breeding through genetic engineering. Here we present a detailed protocol for a highly efficient -mediated transformation method for sweet basil, which was established based on a previously reported method by other researchers, with modifications on several aspects, including growth of sweet basil, age of plants used for explants, preparation and concentration of . This protocol allows researchers in academia and agroindustry to generate transgenic sweet basil plants in an easy, quick and highly reproducible manner. In addition, this protocol may be applicable to transform other species within the genus .
甜罗勒（）是一种具有高经济价值的受欢迎香草，目前正受到一种严重卵菌病害的威胁。高效的转化方法是进行基因功能分析以加速分子育种和部署有效的病害管理策略以及通过基因工程进行育种的先决条件。在此，我们展示了一种针对甜罗勒的高效介导转化方法的详细方案，该方案是在其他研究人员先前报道的方法基础上建立的，在几个方面进行了改进，包括甜罗勒的生长、用于外植体的植株年龄、的制备和浓度。该方案使学术界和农业产业的研究人员能够以简便、快速且高度可重复的方式培育转基因甜罗勒植株。此外，该方案可能适用于转化该属内的其他物种。
