Ph.D. Program in Biomedical Engineering, Chang Gung University, Taoyuan City 33302, Taiwan.
Division of Haematology/Oncology, Department of Internal Medicine, New Taipei Municipal Hospital, New Taipei City 23600, Taiwan.
Biosensors (Basel). 2020 Jun 16;10(6):65. doi: 10.3390/bios10060065.
Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed-Whether, the ODEP field affects the native properties of cells. To address this issue, we explored the effect of ODEP electrical conditions on cellular properties. Within the experimental conditions tested, the ODEP-based cell manipulation with the largest velocity occurred at 10 Vpp and 1 MHz, for the two cancer cell types explored. Under this operating condition, however, the cell viability of cancer cells was significantly affected (e.g., 70.5 ± 10.0% and 50.6 ± 9.2% reduction for the PC-3 and SK-BR-3 cancer cells, respectively). Conversely, the exposure of cancer cells to the ODEP electrical conditions of 7-10 Vpp and 3-5 MHz did not significantly alter the cell viability, cell metabolic activity, and the , , and gene expression of cancer cells. Overall, this study fundamentally investigated the effect of ODEP electrical conditions on the cellular properties of cancer cells. The information obtained is crucially important for the utilization of ODEP-based cell manipulation in a microscale system for various applications.
细胞在微流控系统中使用光诱导介电泳（ODEP）进行操纵因其简单而引起了科学家们的兴趣。尽管该技术已成功应用于各种应用，但是必须解决一个基本问题——ODEP 场是否会影响细胞的固有特性。为了解决这个问题，我们研究了 ODEP 电气条件对细胞特性的影响。在所测试的实验条件下，对于所研究的两种癌细胞类型，基于 ODEP 的最大细胞速度出现在 10 Vpp 和 1 MHz。然而，在这种工作条件下，癌细胞的存活率受到了显著影响（例如，PC-3 和 SK-BR-3 癌细胞的存活率分别降低了 70.5±10.0%和 50.6±9.2%）。相反，癌细胞暴露于 7-10 Vpp 和 3-5 MHz 的 ODEP 电气条件下不会显著改变癌细胞的存活率、细胞代谢活性以及 、 和 基因的表达。总的来说，本研究从根本上研究了 ODEP 电气条件对癌细胞的细胞特性的影响。所获得的信息对于在微尺度系统中基于 ODEP 的细胞操纵在各种应用中的利用至关重要。
