Lima Maíra A, Silva Suély V, Jaeger Ruy G, Freitas Vanessa M
Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1524, Ed Biomédicas 1 sala 428, São Paulo, SP 05508-000, Brazil.
Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1524, Ed Biomédicas 1 sala 428, São Paulo, SP 05508-000, Brazil.
Steroids. 2020 Sep;161:108680. doi: 10.1016/j.steroids.2020.108680. Epub 2020 Jun 18.
The progression of cancer depends on the interaction between the cells and their microenvironment. Progesterone is a steroid and progestogen sex hormone produced by the corpus luteum, which is a transitory endocrine gland in female mammals and prepares the endometrium for implantation. Also, progesterone is involved in antitumorigenic process in different types of cancer. Our goal is to investigate the role of progesterone in cell invasion and migration. Ovarian cells were treated with different concentrations of progesterone. 500 nM or 1 μM progesterone decreased the migration of the cells in 24 h or less without affecting the viability. Immunoblot showed that treatment with 1 μM progesterone decreased the phosphorylated forms of Src and FAK, and the cells were less polarized. Our results suggest that progesterone interferes with migration and invasion of ovarian cells. Inhibitory experiments inferred the progesterone receptor playing a role in migration and invasion. Decreased phosphorylation of molecules involved in these processes was also found.
癌症的进展取决于细胞与其微环境之间的相互作用。孕酮是一种类固醇和孕激素性激素，由黄体产生，黄体是雌性哺乳动物中的一种临时性内分泌腺，为子宫内膜着床做准备。此外，孕酮参与不同类型癌症的抗肿瘤过程。我们的目标是研究孕酮在细胞侵袭和迁移中的作用。用不同浓度的孕酮处理卵巢细胞。500 nM或1 μM孕酮在24小时或更短时间内降低了细胞的迁移，且不影响细胞活力。免疫印迹显示，用1 μM孕酮处理可降低Src和FAK的磷酸化形式，且细胞的极化程度降低。我们的结果表明，孕酮会干扰卵巢细胞的迁移和侵袭。抑制实验推断孕酮受体在迁移和侵袭中发挥作用。还发现参与这些过程的分子磷酸化减少。
