术后辅助化疗和姑息化疗对结直肠癌肠道微生物组的影响。

Effects of postoperative adjuvant chemotherapy and palliative chemotherapy on the gut microbiome in colorectal cancer.

机构信息

Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hops HuZhou University. Address: 198 Hongqi Rd, Huzhou, Zhejiang, China.

Department of Intervention and Radiotherapy, Huzhou Central Hospital, Address: No. 198 Hongqi Road, Huzhou, Zhejiang Province, 313000, China.

出版信息

Microb Pathog. 2020 Dec;149:104343. doi: 10.1016/j.micpath.2020.104343. Epub 2020 Jun 17.

DOI:10.1016/j.micpath.2020.104343

PMID:32562813

Abstract

BACKGROUND

The gut microbiome changes are related to the colorectal cancer (CRC). Chemotherapy is one of the main treatment methods for CRC.

PURPOSE

To explore the effect of chemotherapy on the gut bacteria and fungi in CRC.

METHODS

Total of 11 advanced CRC patients treated with the FOLFIRI regimen, 15 postoperative CRC patients treated with the XELOX regimen, and corresponding CRC patients without surgery and chemotherapy were recruited. The 16S ribosomal RNA and ITS sequences were sequenced, and bioinformatics analysis was executed to screen for the distinctive gut microbiome.

RESULTS

The abundances of Veillonella, Humicola, Tremellomycetes and Malassezia were increased in postoperative CRC patients treated with the XELOX regimen. The abundances of Faecalibacterium, Clostridiales, phascolarctobacterium, Humicola and Rhodotorula were decreased, and the abundances of Candida, Magnusiomyces, Tremellomycetes, Dipodascaceae, Saccharomycetales, Malassezia and Lentinula were increased in advanced CRC patients treated with the FOLFIRI regimen. The abundances of Humicola, Rhodotorula, and Magnusiomyces were decreased, and the abundances of Candida, Tremellomycetes, Dipodascaceae, Saccharomycetales, Malassezia and Lentinula were increased in advanced CRC patients treated with the FOLFIRI regimen combined with cetuximab compared with those treated with the FOLFIRI regimen alone.

CONCLUSIONS

The community structure of gut bacteria and fungi changes in chemotherapy on CRCs.

摘要

背景

肠道微生物群的变化与结直肠癌（CRC）有关。化疗是 CRC 的主要治疗方法之一。

目的

探讨化疗对 CRC 肠道细菌和真菌的影响。

方法

共招募了 11 例接受 FOLFIRI 方案治疗的晚期 CRC 患者、15 例接受 XELOX 方案治疗的术后 CRC 患者和相应的无手术和化疗的 CRC 患者。对 16S 核糖体 RNA 和 ITS 序列进行测序，并进行生物信息学分析，以筛选出独特的肠道微生物群。

结果

XELOX 方案治疗的术后 CRC 患者中，韦荣球菌属、高温放线菌属、担子菌纲和马拉色菌属的丰度增加。而 FOLFIRI 方案治疗的晚期 CRC 患者中，粪杆菌属、梭菌目、拟杆菌科、高温放线菌属和红酵母属的丰度降低，而念珠菌属、Magnusiomyces、担子菌纲、毛壳菌科、酵母目、马拉色菌属和香菇属的丰度增加。XELOX 方案联合西妥昔单抗治疗的晚期 CRC 患者中，高温放线菌属、红酵母属和 Magnusiomyces 的丰度降低，而念珠菌属、担子菌纲、毛壳菌科、酵母目、马拉色菌属和香菇属的丰度增加。