College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 201306, China.
College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 201306, China.
Fish Shellfish Immunol. 2020 Sep;104:686-692. doi: 10.1016/j.fsi.2020.05.078. Epub 2020 Jun 17.
Litopenaeus vannamei (Pacific white shrimp) is one of the most commercially important varieties of shrimp cultivated in the world. Shrimp farming is a high-risk, capital-intensive industry that is susceptible to periodic outbreaks of diseases caused by viral and bacterial pathogens. Thus, there is a need to develop economically viable methods of disease control. The hepatopancreas of crustaceans are known to have an important role in their innate immune response. In this study, we have explored the immune response of the hepatopancreas from L. vannamei fed with trans-vp28 gene Synechocystis sp. PCC6803 using iTRAQ-based proteomics. A total of 214 differentially expressed proteins (DEPs) were identified, of which 143 were up-regulated and 71 were down-regulated. These proteins have diverse roles in the cell cytoskeleton and cell phagocytosis, antioxidant defense process and the response of immune related proteins. Among these proteins, the immunity associated with the functional annotation of L. vannamei was further analysed. In addition, 4 DEPs (act1, N/A, H and C7M84_013542) were analysed using parallel reaction monitoring (PRM). This is the first report of proteomics in the hepatopancreas of L. vannamei immunized with trans-vp28 gene Synechocystis sp. PCC6803.
凡纳滨对虾（太平洋白对虾）是世界上养殖最广泛的商业虾种之一。虾类养殖是一个高风险、资本密集型产业，易受到病毒和细菌病原体周期性爆发的影响。因此，需要开发经济可行的疾病控制方法。甲壳类动物的肝胰腺在其先天免疫反应中具有重要作用。在这项研究中，我们使用 iTRAQ 基于蛋白质组学技术研究了喂食转 vp28 基因集胞藻 sp. PCC6803 的凡纳滨对虾肝胰腺的免疫反应。共鉴定出 214 个差异表达蛋白（DEPs），其中 143 个上调，71 个下调。这些蛋白质在细胞骨架和细胞吞噬、抗氧化防御过程以及免疫相关蛋白的反应中具有多种作用。在这些蛋白质中，进一步分析了与凡纳滨对虾功能注释相关的免疫。此外，使用平行反应监测（PRM）分析了 4 个 DEP（act1、N/A、H 和 C7M84_013542）。这是首次报道转 vp28 基因集胞藻 sp. PCC6803 免疫凡纳滨对虾肝胰腺的蛋白质组学研究。
