从大鼠切牙牙髓建立克隆细胞系（RPC-C2A）并进行鉴定。
Establishment and characterization of a clonal cell line (RPC-C2A) from dental pulp of the rat incisor.
作者信息
Kasugai S, Adachi M, Ogura H
机构信息
Department of Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
出版信息
Arch Oral Biol. 1988;33(12):887-91. doi: 10.1016/0003-9969(88)90018-0.
Maxillary incisor pulp cells from male Wistar rats (7 weeks old) were cultured. After the 52nd subculture, cloning was performed twice and a clonal cell line (RPC-C2A) with high alkaline phosphatase (ALP) activity was established. The population doubling time of RPC-C2A cells was 13.7 h, and the mode of the chromosome number was 42. The heat stability of ALP, the effect of the ALP inhibitors, and the ALP polyacrylamide gel electrophoresis pattern in RPC-C2A cells was the same as for the ALP of the isolated dental pulp.
培养来自7周龄雄性Wistar大鼠的上颌切牙牙髓细胞。在第52次传代培养后，进行了两次克隆，并建立了具有高碱性磷酸酶（ALP）活性的克隆细胞系（RPC-C2A）。RPC-C2A细胞的群体倍增时间为13.7小时，染色体数目的众数为42。RPC-C2A细胞中ALP的热稳定性、ALP抑制剂的作用以及ALP聚丙烯酰胺凝胶电泳图谱与分离的牙髓ALP相同。
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