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次氯酸作为龋损牙本质潜在的窝洞调节剂。

Hypochlorous acid as a potential cavity conditioner for caries-affected dentin.

作者信息

Sanon Kittisak, Elseoudy Nader, Hiraishi Noriko, Tamura Yukihiko, Sanchavanakit Neeracha, ThanNaing SoeKayThwe, Shimada Yasushi, Tagami Junji, Thongthai Pasiree

机构信息

Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University, 34 Henri Dunant Road, Pathumwan, Bangkok, 10330, Thailand.

Smart Innovations for Preventive and Restorative Dentistry Research Group, Faculty of Dentistry, Chulalongkorn University, 34 Henri Dunant Road, Pathumwan, Bangkok, 10330, Thailand.

出版信息

Sci Rep. 2025 Jul 1;15(1):21438. doi: 10.1038/s41598-025-06191-7.

Abstract

To evaluate the effects of hypochlorous acid (HOCl) conditioning on bond strength to caries-affected dentin (CAD), antibacterial efficacy against Streptococcus mutans (S. mutans), and dental pulp cell compatibility. Extracted human molars with occlusal caries were pretreated with 50 ppm HOCl before adhesive application (Clearfil SE Bond 2), with untreated specimens serving as controls. Microtensile bond strength (µTBS) was evaluated after 24-hour storage and 10,000 thermocycles. Antibacterial efficacy was assessed by colony-forming unit (CFU) enumeration following S. mutans incubation with HOCl solution. Rat dental pulp cells were cultured with HOCl at concentrations of 0, 0.5, 1, 5, 10, and 50 ppm. Cell viability was assessed using Cell Counting Kit-8. Data underwent statistical analysis using one-way ANOVA with Tukey's post hoc test (α = 0.05). HOCl pretreatment significantly enhanced µTBS to CAD (from 25.6 ± 4.3 to 35.8 ± 7.2, p < 0.001) and maintained bond stability after thermocycling (32.1 ± 4.5, p > 0.05). HOCl demonstrated significant antibacterial efficacy against S. mutans with substantial reduction of viable bacteria (p < 0.001). Additionally, HOCl maintained compatibility with dental pulp cells. HOCl shows a promise as a dentin conditioner, offering enhance bond performance to CAD with improved bond durability, while providing antibacterial benefits and maintaining biocompatibility.

摘要

评估次氯酸(HOCl)预处理对龋损牙本质(CAD)粘结强度、对变形链球菌(S. mutans)的抗菌效果以及牙髓细胞相容性的影响。将有咬合面龋的拔除人类磨牙在应用粘结剂(Clearfil SE Bond 2)前用50 ppm HOCl进行预处理,未处理的标本作为对照。在储存24小时和进行10000次热循环后评估微拉伸粘结强度(µTBS)。通过将变形链球菌与HOCl溶液孵育后的菌落形成单位(CFU)计数来评估抗菌效果。将大鼠牙髓细胞与浓度为0、0.5、1、5、10和50 ppm的HOCl一起培养。使用细胞计数试剂盒-8评估细胞活力。数据采用单因素方差分析和Tukey事后检验进行统计分析(α = 0.05)。HOCl预处理显著提高了对CAD的µTBS(从25.6±4.3提高到35.8±7.2,p < 0.001),并在热循环后保持了粘结稳定性(32.1±4.5,p > 0.05)。HOCl对变形链球菌显示出显著的抗菌效果,活菌数量大幅减少(p < 0.001)。此外,HOCl保持了与牙髓细胞的相容性。HOCl作为一种牙本质预处理剂具有前景,可提高对CAD的粘结性能,改善粘结耐久性,同时提供抗菌益处并保持生物相容性。

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