两步酸催化甘油预处理甘蔗渣生产油脂酵母生物质的放大研究。
Scale-up of two-step acid-catalysed glycerol pretreatment for production of oleaginous yeast biomass from sugarcane bagasse by Rhodosporidium toruloides.
机构信息
Centre for Agriculture and the Bioeconomy, Institute for Future Environments, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia; School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia.
Centre for Agriculture and the Bioeconomy, Institute for Future Environments, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia; School of Biology & Environmental Science, Science and Engineering Faculty, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia.
出版信息
Bioresour Technol. 2020 Oct;313:123666. doi: 10.1016/j.biortech.2020.123666. Epub 2020 Jun 13.
Two-step dilute acid and acid-catalysed glycerol pretreatment was developed to maximise sugar yield from sugarcane bagasse. At the laboratory scale, dilute acid pretreatment at 130 °C followed by acid-catalysed glycerol pretreatment at 170 °C led to a total sugar (C5 + C6) yield of 82%, 31% higher than that from one-step acid-catalysed glycerol pretreatment. At the pilot scale, the two-step dilute acid and acid-catalysed glycerol pretreatment led to a maximum sugar yield of 74%, 13% higher than that from one-step pretreatment with 52% reduction in glycerol usage. The enzymatic hydrolysate containing glucose and residual glycerol were used to produce microbial oils by a Rhodosporidium toruloides strain. A fed-batch cultivation strategy led to the production of 44.8 g/L cell mass, including 26.6 g/L oil, 8.6 g/L protein and 12.7 mg/L carotenoid. The cell mass and oil yields were 19% higher than those from batch cultivation as feedstock inhibition and catabolite repression were alleviated.
两步稀酸和酸催化甘油预处理方法被开发出来，以最大限度地从甘蔗渣中获得糖产量。在实验室规模下，130°C 的稀酸预处理后接着进行 170°C 的酸催化甘油预处理，可得到 82%的总糖（C5+C6）产量，比一步酸催化甘油预处理高 31%。在中试规模下，两步稀酸和酸催化甘油预处理可得到最高 74%的糖产量，比一步预处理高 13%，同时甘油用量减少了 52%。含有葡萄糖和残余甘油的酶解液被用来通过一株 Rhodosporidium toruloides 菌株生产微生物油脂。采用分批补料培养策略，可生产出 44.8 g/L 的细胞质量，其中包括 26.6 g/L 的油脂、8.6 g/L 的蛋白质和 12.7 mg/L 的类胡萝卜素。与分批培养相比，细胞质量和油脂产量提高了 19%，因为减轻了基质抑制和分解代谢物阻遏。
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