Centre for Agriculture and the Bioeconomy, Institute for Future Environments, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia; School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia.
Centre for Agriculture and the Bioeconomy, Institute for Future Environments, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia; School of Biology & Environmental Science, Science and Engineering Faculty, Queensland University of Technology, 2 George St, Brisbane, Qld 4000, Australia.
Bioresour Technol. 2020 Dec;318:124059. doi: 10.1016/j.biortech.2020.124059. Epub 2020 Sep 2.
Acid-catalysed crude glycerol (ACG) pretreatment was carried out at 110 °C and 130 °C for mild fractionation of sugarcane bagasse into fermentable sugars and high-quality lignin. ACG pretreatment at 110 °C led to sugar yields of 71%-74%, comparable to those with acid-catalysed reagent-grade glycerol (AG). ACG pretreatment removed more lignin (53%-75%) than AG pretreatment (38%-49%), likely due to the presence of organic impurities in ACG. Hence, 28% more lignin was recovered from ACG pretreatment hydrolysate than with the AG pretreatment. NMR analysis revealed that recovered lignin was modified by glycerol through etherification of β-aryl ethers and esterification of hydroxycinnamic acids, which prevented lignin condensation and led to the generation of β-O-4 linkage-rich lignin at mild conditions (110 °C for 3 h and 5 h). This study suggests that crude glycerol is a suitable low-cost solvent for mild fractionation of lignocellulosic biomass into fermentable sugars and high-quality lignin for value-adding applications.
酸催化粗甘油（ACG）预处理在 110°C 和 130°C 下进行，将甘蔗渣温和地分馏为可发酵糖和高质量木质素。ACG 预处理在 110°C 下导致糖收率为 71%-74%，可与酸催化试剂级甘油（AG）相媲美。ACG 预处理比 AG 预处理去除了更多的木质素（53%-75%），可能是由于 ACG 中存在有机杂质。因此，从 ACG 预处理水解液中回收的木质素比 AG 预处理多 28%。NMR 分析表明，回收的木质素通过β-芳基醚的醚化和对羟基肉桂酸的酯化被甘油改性，这阻止了木质素缩合，并在温和条件下（110°C 下 3 小时和 5 小时）产生富含β-O-4 键的木质素。本研究表明，粗甘油是一种合适的低成本溶剂，可将木质纤维素生物质温和地分馏为可发酵糖和高质量木质素，用于增值应用。
