溃疡性结肠炎患者粪便微生物群移植后细胞因子变化评估的初步研究。
Pilot study of cytokine changes evaluation after fecal microbiota transplantation in patients with ulcerative colitis.
机构信息
Department of Gastroenterology and Hepatology, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China; Medical Laboratory Center, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.
Department of Gastroenterology and Hepatology, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.
出版信息
Int Immunopharmacol. 2020 Aug;85:106661. doi: 10.1016/j.intimp.2020.106661. Epub 2020 Jun 17.
AIMS
To evaluate the changes of serum cytokines levels after fecal microbiota transplantation (FMT) in patients with active ulcerative colitis (UC) and the correlation with UC disease activity.
METHODS
Patients with active UC who meet the inclusion and exclusion criteria were recruited, and received FMT from a single donor for three times with an interval of 2-3 months. Serum samples were collected before every FMT. Clinical responses to FMT were assessed according to the criteria of Mayo score. 41 serum cytokines, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were quantitatively detected. Changes in serum cytokines levels after FMT and their correlation with CRP, ESR and Mayo scores were investigated.
RESULTS
16 active UC patients were enrolled, and 14(87.5%) patients achieved clinical response to FMT. Compared with those before FMT, serum concentrations of IL-1Ra, IL-6, IP-10 and ENA-78 decreased significantly after the second FMT (P < 0.05), and serum concentrations of MEC, VCAM-1 and G-CSF decreased significantly after both the first and second FMT (P < 0.05). Serum levels of IL-6, IL-1Ra and VCAM-1 were all significantly positively correlated with CRP and ESR. Serum level of IP-10 was significantly positively correlated with CRP, ESR and Mayo score. Serum level of G-CSF was significantly positively correlated with Mayo score.
CONCLUSIONS
FMT may play a therapeutic role partly through modulating the host immune response. IL-6, IL-1Ra, IP-10, VCAM-1 and G-CSF may be biomarkers to evaluate the effect of FMT on UC.
目的
评估粪便微生物群移植（FMT）后活动期溃疡性结肠炎（UC）患者血清细胞因子水平的变化及其与 UC 疾病活动度的相关性。
方法
符合纳入排除标准的活动期 UC 患者，接受来自单一供体的 FMT 三次，间隔 2-3 个月。每次 FMT 前采集血清样本。根据 Mayo 评分标准评估 FMT 的临床反应。定量检测 41 种血清细胞因子、C 反应蛋白（CRP）和红细胞沉降率（ESR）。研究 FMT 后血清细胞因子水平的变化及其与 CRP、ESR 和 Mayo 评分的相关性。
结果
共纳入 16 例活动期 UC 患者，14 例（87.5%）患者对 FMT 有临床反应。与 FMT 前相比，第二次 FMT 后血清 IL-1Ra、IL-6、IP-10 和 ENA-78 浓度显著降低（P<0.05），第一次和第二次 FMT 后血清 MEC、VCAM-1 和 G-CSF 浓度显著降低（P<0.05）。血清 IL-6、IL-1Ra 和 VCAM-1 水平均与 CRP 和 ESR 呈显著正相关。血清 IP-10 水平与 CRP、ESR 和 Mayo 评分呈显著正相关。血清 G-CSF 水平与 Mayo 评分呈显著正相关。
结论
FMT 可能部分通过调节宿主免疫反应发挥治疗作用。IL-6、IL-1Ra、IP-10、VCAM-1 和 G-CSF 可能是评估 FMT 对 UC 疗效的生物标志物。
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