Department of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian, 116600, China.
Department of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian, 116600, China.
J Pharm Biomed Anal. 2020 Sep 10;189:113416. doi: 10.1016/j.jpba.2020.113416. Epub 2020 Jun 11.
Processing herbal medicine is a classic characters of traditional Chinese medicine (TCM) clinic. Stir-frying with bran is a common method of processing Atractylodis Rhizoma (AR), which is considered to enhance the therapeutic effect in TCM theory. However, the processing mechanism remains unclear. In our previous study, we found that bran-fried AR was better than raw AR in regulating gut flora. To further compare the effect of raw and bran-fried AR on the symbiotic metabolism of gut flora and host, we established a fecal metabonomics method of ARs intervention on spleen-deficiency rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. 22 potential biomarkers of spleen-deficiency were identified under the positive and negative ion mode. Through the analysis of metabolic pathway, 6 significant changes of metabolic pathway were found, 5 of which were both improved by raw and bran-fried AR. Especially bran-fried AR was better than raw AR in the improvement of tyrosine metabolism and purine metabolism. Correlation analysis between the metabolic potential biomarkers and the microbiological biomarkers published in our previous study revealed that one of the spleen-strengthening mechanisms of ARs could be associated with the improvement of the symbiotic metabolism of gut flora and host by regulating gut-flora. The results can help us to understand the mechanism of AR intervention on spleen deficiency and the enhanced-efficacy mechanism of bran-fried AR.
炮制中药是中医临床的经典特色。麸炒是白术常用的炮制方法，在中医理论中认为麸炒可以增强疗效。然而，其炮制机制尚不清楚。在我们之前的研究中，我们发现麸炒白术比生白术更能调节肠道菌群。为了进一步比较生白术和麸炒白术对肠道菌群与宿主共生代谢的影响，我们建立了超高效液相色谱-四极杆飞行时间质谱联用的白术干预脾气虚大鼠粪便代谢组学方法。在正、负离子模式下共鉴定出 22 个脾气虚潜在生物标志物。通过代谢途径分析，发现 6 条代谢途径发生了显著变化，其中 5 条均被生白术和麸炒白术改善。特别是麸炒白术在改善酪氨酸代谢和嘌呤代谢方面优于生白术。对本研究中代谢潜在生物标志物与我们之前发表的微生物生物标志物的相关性分析表明，白术的健脾机制之一可能与调节肠道菌群共生代谢有关。该结果有助于我们了解白术干预脾气虚的作用机制和麸炒白术增效的机制。
