加味芍药汤通过抑制炎症减轻右旋糖酐硫酸钠诱导的溃疡性结肠炎。

Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation.

作者信息

Qiao Huixia, Huang Yahui, Chen Xiaoyan, Yang Long, Wang Yue, Xu Rongrong

机构信息

Department of Digestive, Xi'an Traditional Chinese Medicine Hospital, No. 69, Fengcheng Eight Road, Weiyang District, Xi'an City, Shaanxi Province 710021, China.

出版信息

Gastroenterol Res Pract. 2020 May 27;2020:7182874. doi: 10.1155/2020/7182874. eCollection 2020.

PURPOSE

Jiaweishaoyao decoction (JWSYD) is a traditional prescription of Chinese medicine that is initially used for the treatment of diarrhea. This study is aimed at investigating the effects of JWSYD on DSS-induced ulcerative colitis (UC).

METHODS

DSS-induced UC mice and LPS-induced RAW264.7 cells were used as the UC model and . UC was assessed by body weight, disease activity index (DAI), colon length, spleen weight, and histopathological score (HE staining). The levels of TNF-, IL-1, and IL-6 were analyzed by ELISA and qRT-PCR. The levels of NLRP3 inflammasome- and NF-B pathway-associated proteins were measured by western blot.

RESULTS

JWSYD alleviated DSS-induced UC in respect to body weight, DAI, colon length, spleen weight, and histopathological score. JWSYD reduced the levels of TNF-, IL-1, and IL-6 in DSS-induced UC mice and the supernatants of LPS-induced RAW264.7 cells. JWSYD suppressed the protein levels of inflammasome-associated proteins, including NLRP3, ASC1, Procaspase-1, Cleaved caspase-1, and Cleaved IL-1 in DSS-induced UC mice and LPS-induced RAW264.7 cells. In addition, JWSYD suppressed the NF-B pathway and .

CONCLUSION

JWSYD alleviated DSS-induced UC via inhibiting the NLRP3 inflammasome and NF-B pathway.

目的

加味芍药汤（JWSYD）是一种传统中药方剂，最初用于治疗腹泻。本研究旨在探讨加味芍药汤对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）的影响。

方法

将DSS诱导的UC小鼠和脂多糖（LPS）诱导的RAW264.7细胞用作UC模型。通过体重、疾病活动指数（DAI）、结肠长度、脾脏重量和组织病理学评分（苏木精-伊红染色）评估UC。采用酶联免疫吸附测定（ELISA）和定量逆转录聚合酶链反应（qRT-PCR）分析肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）的水平。通过蛋白质免疫印迹法检测NLRP3炎性小体和核因子-κB（NF-κB）通路相关蛋白的水平。

结果

加味芍药汤在体重、DAI、结肠长度、脾脏重量和组织病理学评分方面减轻了DSS诱导的UC。加味芍药汤降低了DSS诱导的UC小鼠以及LPS诱导的RAW264.7细胞上清液中TNF-α、IL-1和IL-6的水平。加味芍药汤抑制了DSS诱导的UC小鼠和LPS诱导的RAW264.7细胞中炎性小体相关蛋白的水平，包括NLRP3、凋亡相关斑点样蛋白（ASC1）、半胱天冬酶原-1、裂解的半胱天冬酶-1和裂解的IL-1。此外，加味芍药汤抑制了NF-κB通路。