Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, Salamanca 37007, Spain.
Animal Science Faculty, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogotá 111166, Colombia.
Dis Markers. 2020 May 31;2020:5265198. doi: 10.1155/2020/5265198. eCollection 2020.
Human strongyloidiasis a soil-transmitted infection caused by is one of the most neglected amongst the so-called Neglected Tropical Diseases (NTDs). is a nematode, which is distributed worldwide; it has been estimated that it could affect millions of people, mainly in tropical and subtropical endemic regions. The difficulties of diagnosis lead to infection rates being underreported. Asymptomatic patients have chronic infections that can lead to severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. Strongyloidiasis can easily be misdiagnosed because conventional faecal-based techniques lack of sensitivity for the morphological identification of infective larvae in faeces. None of the currently used molecular methods have used urine samples as an alternative to faecal samples for diagnosing strongyloidiasis. This study was thus aimed at comparing, for the first time, the use of a new loop-mediated isothermal amplification (LAMP) molecular assay (LAMP) to traditional methods on patients' urine samples. Twenty-four urine samples were taken from patients included in a study involving two Spanish hospitals for strongyloidiasis screening using parasitological and serological tests. larvae were found in 11 patients' faecal samples, thereby ascertaining that they had the disease. Other patients had high antibody titres but no larvae were found in their faeces. All urine samples were analysed by PCR and LAMP assay. No amplification occurred when using PCR. LAMP led to detecting DNA in urine samples from patients having previously confirmed strongyloidiasis by parasitological tests and/or a suspicion of being infected by serological ones. The -LAMP assay is a useful molecular tool for research regarding strongyloidiasis in human urine samples. After further validation, the -LAMP assay could also be used for complementary and effective diagnosis of strongyloidiasis in a clinical setting.
人旋毛虫病是一种由 引起的土壤传播感染，是所谓的被忽视热带病（NTDs）中最被忽视的一种。 是一种分布于全球的线虫，据估计可能影响数百万人，主要集中在热带和亚热带流行地区。由于诊断困难，感染率被低估。无症状患者存在慢性感染，可能导致严重的超感染综合征或免疫功能低下患者的播散性旋毛虫病。旋毛虫病很容易被误诊，因为传统的基于粪便的技术缺乏对粪便中感染性幼虫的形态学鉴定的敏感性。目前使用的分子方法都没有将尿液样本用作粪便样本的替代物来诊断旋毛虫病。因此，本研究首次旨在比较一种新的环介导等温扩增（LAMP）分子检测方法（LAMP）与传统方法在患者尿液样本中的应用。从参与西班牙两家医院进行旋毛虫病筛查的研究中的 24 名患者中采集了 24 份尿液样本，使用寄生虫学和血清学检测方法。在 11 名患者的粪便样本中发现了 幼虫，从而确定他们患有该病。其他患者的抗体滴度较高，但粪便中未发现幼虫。对所有尿液样本进行了 PCR 和 LAMP 检测。PCR 未扩增。LAMP 检测到先前通过寄生虫学检测和/或血清学检测怀疑感染的患者的尿液样本中的 DNA。-LAMP 检测是一种用于研究人类尿液样本中旋毛虫病的有用的分子工具。经过进一步验证，-LAMP 检测也可用于临床环境中旋毛虫病的补充和有效诊断。
