Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Methods Mol Biol. 2022;2454:657-664. doi: 10.1007/7651_2020_299.
The characteristics of pluripotent cells have great potential for basic and clinical research and application. We describe the effect of normoxia or hypoxia regarding the proliferation and pluripotency of human iPS cells using colony number counting and real-time polymerase chain reaction (PCR). In addition, the function of hypoxia-inducible factors (HIFs) in human iPS cells under hypoxic conditions is evaluated in relation to the expression of pluripotency markers by siRNA and real-time PCR. Furthermore, we introduce the change of HIF-2α expression when signal transducer and activator of transcription 3 (STAT3) is suppressed by its inhibitor, Stattic or S31 201, using RT-PCR.
多能细胞的特性对于基础和临床研究及应用具有巨大的潜力。我们通过集落计数和实时聚合酶链反应（PCR）描述了常氧或低氧对人诱导多能干细胞增殖和多能性的影响。此外，通过 siRNA 和实时 PCR 评估了低氧条件下缺氧诱导因子（HIFs）在人诱导多能干细胞中的功能与多能性标志物的表达。此外，我们还通过 RT-PCR 介绍了当信号转导和转录激活因子 3（STAT3）被其抑制剂 Stattic 或 S31 201 抑制时，HIF-2α 表达的变化。
