Sugimoto K, Hayashi Yoshihiko
Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, Japan.
Methods Mol Biol. 2016;1357:465-74. doi: 10.1007/7651_2014_158.
iPS cells are attractive for the regenerative medicine. The creation of pluripotent cells from somatic cells has great potential for basic and clinical research and application. Retroviral transduction of four or three transfection factors has been shown to initiate a reprogramming process. Here, we describe the effect of transcription factors regarding the growth and differentiation of mouse iPS cells in normoxia or hypoxia. Furthermore, we introduce the function of hypoxia-inducible factors (HIFs) in mouse iPS cells in hypoxia using RT-PCR and western blotting together with HIFs knockdown techniques.
诱导多能干细胞（iPS细胞）在再生医学领域颇具吸引力。由体细胞产生多能细胞在基础研究、临床研究及应用方面具有巨大潜力。已证实，通过逆转录病毒转导四种或三种转录因子可启动重编程过程。在此，我们描述了转录因子在常氧或低氧条件下对小鼠iPS细胞生长和分化的影响。此外，我们运用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法以及缺氧诱导因子（HIFs）敲低技术，介绍了低氧条件下HIFs在小鼠iPS细胞中的功能。
