Rana Rashmi, Joon Shikha, Kumar Jain Arun, Kumar Mohanty Nayan
Department of Research, Sir Ganga Ram Hospital, New Delhi, India.
National Institute of Pathology, ICMR, New Delhi, India.
Andrologia. 2020 Oct;52(9):e13720. doi: 10.1111/and.13720. Epub 2020 Jun 21.
Phthalate plasticisers in medical, cosmetic and consumer products might pose serious health implications in humans including infertility. We sought to investigate the correlation, if any, between the phthalates and their metabolites and sperm quality parameters, and male infertility. Phthalate esters (15) and their metabolites (5) were estimated in the blood serum and urine samples from the age-matched 152 infertile and 75 fertile males using gas chromatography (GC) and high-performance liquid chromatography (HPLC). Finally, the data were analysed to correlate phthalate exposure and semen quality parameters in the infertility group. The estimated levels of DEHP, DBP, DIBP, BEHIP, BPBG, DPP, DIOP, DIHP, DMP, DINP, BIOP, DMOP and DICHP were significantly higher in the infertile males compared to the fertile males (p < .05 or p < .01). However, these were not found to be associated with the semen quality parameters (sperm count, motility and sperm morphology). Similarly, HPLC data revealed that the associations between semen parameters (sperm count, sperm motility and sperm morphology) and phthalate metabolite (MEHP and MBP) concentrations in urine samples from the infertile males were mostly unremarkable or statistically nonsignificant. Conclusively, environmental exposure to phthalates and their impacts on male infertility were statistically insignificant in our study groups.
医疗、化妆品及消费品中的邻苯二甲酸酯类增塑剂可能会对人类健康造成严重影响，包括导致不育。我们试图研究邻苯二甲酸酯及其代谢物与精子质量参数以及男性不育之间是否存在相关性。我们使用气相色谱法（GC）和高效液相色谱法（HPLC）对152名年龄匹配的不育男性和75名生育能力正常男性的血清和尿液样本中的15种邻苯二甲酸酯及其5种代谢物进行了测定。最后，对数据进行分析，以关联不育组中邻苯二甲酸酯暴露情况与精液质量参数。与生育能力正常的男性相比，不育男性中邻苯二甲酸二（2-乙基己基）酯（DEHP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸二异丁酯（DIBP）、双（2-乙基己基）邻苯二甲酸酯（BEHIP）、邻苯二甲酸丁苄酯（BPBG）、邻苯二甲酸二戊酯（DPP）、邻苯二甲酸二异辛酯（DIOP）、邻苯二甲酸二异壬酯（DIHP）、邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二异壬酯（DINP）、双（2-甲氧基乙基）邻苯二甲酸酯（BIOP）、邻苯二甲酸二（2-甲氧基乙基）酯（DMOP）和邻苯二甲酸二（2-氯乙基）酯（DICHP）的估计水平显著更高（p < 0.05或p < 0.01）。然而，未发现这些物质与精液质量参数（精子数量、活力和精子形态）有关。同样，HPLC数据显示，不育男性尿液样本中精液参数（精子数量、精子活力和精子形态）与邻苯二甲酸酯代谢物（单-（2-乙基己基）邻苯二甲酸酯（MEHP）和单苄基邻苯二甲酸酯（MBP））浓度之间的关联大多不显著或无统计学意义。总之，在我们的研究组中，环境中邻苯二甲酸酯暴露及其对男性不育的影响在统计学上不显著。
