Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, Arcavacata di Rende, I87036, Italy.
J Mass Spectrom. 2020 Sep;55(9):e4526. doi: 10.1002/jms.4526.
A fast methodology for the assay of parabens in drug and cosmetic preparations has been presented. The procedure developed is based on paper spray tandem mass spectrometry and isotope dilution approach. For each investigated paraben, the corresponding labeled standard has been used in order to improve the accuracy and reproducibility of the analyses. The MS experiments have been performed under MRM conditions, monitoring the transitions [M-H] → m/z 92 and [M-H] → m/z 98, respectively, for each analyte and the corresponding labeled internal standard. The quantitative assay has been performed using a calibration curve built from 2 to 15 mg/L. The method accuracy, in all case near 100%, was evaluated using fortified samples at two concentration levels, which are representative of the lower and the higher portion of calibration curve. The good values of LOQ, LOD, and reproducibility confirm the consistency of the developed approach.
已经提出了一种快速测定药物和化妆品制剂中对羟基苯甲酸酯的方法。所开发的方法基于纸喷雾串联质谱和同位素稀释法。对于每个研究的对羟基苯甲酸酯，都使用相应的标记标准品，以提高分析的准确性和重现性。MS 实验在 MRM 条件下进行，分别监测每个分析物和相应标记内标物的 [M-H] → m/z 92 和 [M-H] → m/z 98 的跃迁。使用从 2 到 15mg/L 构建的校准曲线进行定量分析。方法的准确度在所有情况下都接近 100%，使用在两个浓度水平下（代表校准曲线的较低和较高部分）进行了加标样品的评估。LOQ、LOD 和重现性的良好值证实了所开发方法的一致性。
