Medical School, Aristotle University of Thessaloniki, Thessaloniki. Greece.
Laboratory of Pathology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki. Greece.
Cir Cir. 2020;88(4):410-419. doi: 10.24875/CIRU.20000084.
The objective of the study was to estimate the effects of synbiotics on laboratory, macroscopic, and histopathologic features in dextran sulfate sodium (DSS) experimental colitis.
A total of 40 Wistar rats received 5% of DSS in their drinking water for 8 days to induce ulcerative colitis (UC). Eight rats were sacrificed to confirm the presence of UC. The remaining rats were randomly assigned to two groups: the synbiotics group, which received synbiotics once per day and the control group, which received tap water for another 8 days.
On the 8 day of DSS administration animals developed UC with bloody diarrhea. In the majority of the hematologic variables studied (hemoglobin [HB], red blood cells, platelets, mean corpuscular volume, and mean corpuscular HB), in bodyweight and histopathologic colitis score there was no significant difference between groups. However, the synbiotics group, compared to control, presented a significantly greater colon length on the 4 day, significantly increased hematocrit (HT) on the 8 day, and a significantly decreased number of myeloperoxidase positive cells on the 8 day. Furthermore, there was a trend toward histopathological and clinical improvement.
Administration of synbiotics in the experimental UC results in an attenuation of mucosal inflammatory neutrophil infiltration and an increase in HT.
本研究旨在评估合生剂对葡聚糖硫酸钠（DSS）实验性结肠炎的实验室、宏观和组织病理学特征的影响。
共 40 只 Wistar 大鼠饮用 5%的 DSS 水 8 天以诱导溃疡性结肠炎（UC）。8 只大鼠被处死以确认 UC 的存在。其余大鼠被随机分为两组：合生剂组，每天接受合生剂一次；对照组，再接受自来水 8 天。
在 DSS 给药的第 8 天，动物出现带血腹泻的 UC。在研究的大多数血液学变量（血红蛋白[HB]、红细胞、血小板、平均红细胞体积和平均红细胞 HB）、体重和组织病理学结肠炎评分中，两组之间无显著差异。然而，与对照组相比，合生剂组在第 4 天的结肠长度显著增加，第 8 天的红细胞压积（HT）显著增加，第 8 天的髓过氧化物酶阳性细胞数量显著减少。此外，组织病理学和临床改善呈趋势。
在实验性 UC 中给予合生剂可减轻黏膜炎症性中性粒细胞浸润，并增加 HT。
