Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Nutrients. 2022 Jan 5;14(1):227. doi: 10.3390/nu14010227.
This study aimed to investigate the protective effect of probiotics and synbiotics from traditional Thai fermented tea leaves (Miang) on dextran sulfate sodium (DSS)-induced colitis in mice, in comparison to sulfasalazine. C57BL/6 mice were treated with probiotics A14-6, CMY46 and synbiotics, A14-6 combined with XOS, and CMY46 combined with GOS for 21 days. Colitis was induced with 2% DSS administration for seven days during the last seven days of the experimental period. The positive group was treated with sulfasalazine. At the end of the experiment, clinical symptoms, pathohistological changes, intestinal barrier integrity, and inflammatory markers were analyzed. The probiotics and synbiotics from Miang ameliorated DSS-induced colitis by protecting body weight loss, decreasing disease activity index, restoring the colon length, and reducing pathohistological damages. Furthermore, treatment with probiotics and synbiotics improved intestinal barrier integrity, accompanied by lowing colonic and systemic inflammation. In addition, synbiotics CMY46 combined with GOS remarkedly elevated the expression of IL-10. These results suggested that synbiotics isolated from Miang had more effectiveness than sulfasalazine. Thereby, they could represent a novel potential natural agent against colonic inflammation.
本研究旨在探讨传统泰国发酵茶叶（Miang）中的益生菌和合生菌对葡聚糖硫酸钠（DSS）诱导的结肠炎的保护作用，并与柳氮磺胺吡啶进行比较。C57BL/6 小鼠用益生菌 A14-6、CMY46 和合生菌 A14-6 与 XOS 结合物、CMY46 与 GOS 结合物进行 21 天的处理。在实验的最后七天中，用 2%的 DSS 进行七天的处理以诱导结肠炎。阳性组用柳氮磺胺吡啶治疗。实验结束时，分析临床症状、组织病理学变化、肠道屏障完整性和炎症标志物。Miang 的益生菌和合生菌通过保护体重减轻、降低疾病活动指数、恢复结肠长度和减少组织病理学损伤来改善 DSS 诱导的结肠炎。此外，益生菌和合生菌治疗可改善肠道屏障完整性，同时降低结肠和全身炎症。此外，合生菌 CMY46 与 GOS 的联合使用显著提高了 IL-10 的表达。这些结果表明，Miang 分离的合生菌比柳氮磺胺吡啶更有效。因此，它们可能代表一种针对结肠炎症的新型潜在天然药物。
