Department of Food Technology, Safety and Health, Ghent University, Coupure links 653, 9000 Ghent, Belgium.
Center for Microbial Ecology and Technology, Ghent University, Coupure links 653, 9000 Ghent, Belgium.
FEMS Microbiol Ecol. 2020 Jul 1;96(7). doi: 10.1093/femsec/fiaa105.
Undernutrition remains a public health problem in the developing world with an attributable under-five death proportion of 45%. Lower gut microbiota diversity and poor metabolic output are associated with undernutrition and new therapeutic paths may come from steering gut microbiota composition and functionality. Using a dynamic gut model, the Simulator of Human Intestinal Microbial Ecosystem (SHIME®), we investigated the effect of a lipid-based nutrient supplement enriched with prebiotics (LNSp), compared to LNS alone and control treatment, on the composition and metabolic functionality of fecal microbiota from three infants suffering from undernutrition. LNS elicited a significant increase in acetate and branched-chain fatty acid production, and a higher relative abundance of the genera Prevotella, Megasphaera, Acinetobacter, Acidaminococcus and Pseudomonas. In contrast, LNSp treatment resulted in a significant 9-fold increase in Bifidobacterium relative abundance and a decrease in that of potential pathogens and detrimental bacteria such as Enterobacteriaceae spp. and Bilophila sp. Moreover, the LNSp treatment resulted in a significantly higher production of acetate, butyrate and propionate, as compared to control and LNS. Our results suggest that provision of prebiotic-enhanced LNS to undernourished children could be a possible strategy to steer the microbiota toward a more beneficial composition and metabolic activity. Further in vivo investigations are needed to assess these effects and their repercussion on nutritional status.
营养不良仍然是发展中国家的一个公共卫生问题，可归因于五岁以下儿童死亡的比例为 45%。较低的肠道微生物多样性和较差的代谢产物与营养不良有关，新的治疗途径可能来自于引导肠道微生物群落组成和功能。我们使用动态肠道模型——人类肠道微生物生态系统模拟器（SHIME®），研究了富含益生元的脂质营养素补充剂（LNSp）与 LNS 单独使用和对照处理相比，对 3 名营养不良婴儿粪便微生物群落组成和代谢功能的影响。LNS 引起了乙酸和支链脂肪酸产生的显著增加，以及Prevotella、Megasphaera、Acinetobacter、Acidaminococcus 和 Pseudomonas 属的相对丰度更高。相比之下，LNSp 处理导致双歧杆菌相对丰度显著增加 9 倍，同时减少了潜在的病原体和有害细菌，如肠杆菌科和Bilophila 属。此外，与对照和 LNS 相比，LNSp 处理导致乙酸、丁酸和丙酸的产量显著增加。我们的结果表明，为营养不良的儿童提供富含益生元的 LNS 可能是一种引导微生物群落向更有益的组成和代谢活性发展的策略。需要进一步的体内研究来评估这些效果及其对营养状况的影响。
